Small-angle scattering as a tool to study the thermal denaturation of DNA

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dc.contributor.authorWood, Ken_AU
dc.contributor.authorKnott, RBen_AU
dc.contributor.authorTonchev, Oen_AU
dc.contributor.authorAngelov, Den_AU
dc.contributor.authorTheodorakopoulos, Nen_AU
dc.contributor.authorPeyrard, Men_AU
dc.date.accessioned2016-12-12T01:14:26Zen_AU
dc.date.available2016-12-12T01:14:26Zen_AU
dc.date.issued2014-10-02en_AU
dc.date.statistics2016-12-12en_AU
dc.description.abstractDNA thermal denaturation is the breaking of the base pairs, leading to a splitting of the two strands of the double helix. While it is easy to measure the fraction of open base pairs $(f)$ vs. temperature, determining the fraction $(p)$ of fully open molecules is much harder. Previously, the simultaneous recording of f and p could only be achieved for special sequences. We show that small-angle scattering of X-rays or neutrons allows the measurement of p for any sequence. We illustrate the method with a SAXS investigation of two sequences designed to exhibit different melting profiles and compare the SAXS data with nano-calorimetric measurements of the melting curve. © 2014, EPLA.en_AU
dc.identifier.articlenumber18002en_AU
dc.identifier.citationWood, K., Knott, R., Tonchev, O., Angelov, D., Theodorakopoulos, N., & Peyrard, M. (2014). Small-angle scattering as a tool to study the thermal denaturation of DNA. Europhysics Letters, 108(1), 18002. doi:10.1209/0295-5075/108/18002en_AU
dc.identifier.govdoc7690en_AU
dc.identifier.issn1286-4854en_AU
dc.identifier.issue1en_AU
dc.identifier.journaltitleEurophysics Lettersen_AU
dc.identifier.urihttp://dx.doi.org/10.1209/0295-5075/108/18002en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/8147en_AU
dc.identifier.volume108en_AU
dc.language.isoenen_AU
dc.publisherIOP Scienceen_AU
dc.subjectDNAen_AU
dc.subjectTemperature rangeen_AU
dc.subjectMoleculesen_AU
dc.subjectX-ray equipmenten_AU
dc.subjectDataen_AU
dc.subjectCalorimetryen_AU
dc.titleSmall-angle scattering as a tool to study the thermal denaturation of DNAen_AU
dc.typeJournal Articleen_AU
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