Human serum albumin binding to silica nanoparticles - effect of protein fatty acid ligand

Simple item page
dc.contributor.authorAng, JCen_AU
dc.contributor.authorHenderson, MJen_AU
dc.contributor.authorCampbell, RAen_AU
dc.contributor.authorLin, JMen_AU
dc.contributor.authorYaron, PNen_AU
dc.contributor.authorNelson, Aen_AU
dc.contributor.authorWhite, JWen_AU
dc.date.accessioned2015-12-28T00:50:23Zen_AU
dc.date.available2015-12-28T00:50:23Zen_AU
dc.date.issued2014-02-19en_AU
dc.date.statistics2015-12-19en_AU
dc.description.abstractNeutron reflectivity shows that fatted (F-HSA) and defatted (DF-HSA) versions of human serum albumin behave differently in their interaction with silica nanoparticles premixed in buffer solutions although these proteins have close to the same surface excess when the silica is absent. In both cases a silica containing film is quickly established at the air-water interface. This film is stable for F-HSA at all relative protein-silica concentrations measured. This behaviour has been verified for two small silica nanoparticle radii (42 Å and 48 Å). Contrast variation and co-refinement have been used to find the film composition for the F-HSA-silica system. The film structure changes with protein concentration only for the DF-HSA-silica system. The different behaviour of the two proteins is interpreted as a combination of three factors: increased structural stability of F-HSA induced by the fatty acid ligand, differences in the electrostatic interactions, and the higher propensity of defatted albumin to self-aggregate. The interfacial structures of the proteins alone in buffer are also reported and discussed. © 2015, Royal Society of Chemistry.en_AU
dc.identifier.citationAng, J. C., Henderson, M. J., Campbell, R. A., Lin, J.-M., Yaron, P. N., Nelson, A., Faunce, J., & White, J. W. (2014). Human serum albumin binding to silica nanoparticles - effect of protein fatty acid ligand. Physical Chemistry Chemical Physics, 16, 10157-10168. doi:10.1039/c4cp00293hen_AU
dc.identifier.govdoc6348en_AU
dc.identifier.issn1463-9084en_AU
dc.identifier.journaltitlePhysical Chemistry Chemical Physicsen_AU
dc.identifier.pagination10157-10168en_AU
dc.identifier.urihttps://pubs.rsc.org/en/content/articlehtml/2014/cp/c4cp00293hen_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/6481en_AU
dc.identifier.volume16en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectNeutron reflectorsen_AU
dc.subjectAlbuminsen_AU
dc.subjectSilicaen_AU
dc.subjectFilmsen_AU
dc.subjectProteinsen_AU
dc.subjectLigandsen_AU
dc.titleHuman serum albumin binding to silica nanoparticles - effect of protein fatty acid liganden_AU
dc.typeJournal Articleen_AU
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal Articles