Detection of apoptotic cell death in the thymus of dexamethasone treated rats using [123I]Annexin V and in situ oligonucleotide ligation

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dc.contributor.authorZavitsanou, Ken_AU
dc.contributor.authorNguyen, VHen_AU
dc.contributor.authorGreguric, Ien_AU
dc.contributor.authorChapman, Jen_AU
dc.contributor.authorBallantyne, Pen_AU
dc.contributor.authorKatsifis, Aen_AU
dc.date.accessioned2018-09-21T02:31:23Zen_AU
dc.date.available2018-09-21T02:31:23Zen_AU
dc.date.issued2007-06-29en_AU
dc.date.statistics2018-09-20en_AU
dc.description.abstractIn the present study we aimed to establish an animal model of dexamethasone (DEX)-induced apoptosis in the thymus of rats. The degree of apoptosis was determined in the same animals at 6 and 11 h after a single administration of DEX (5 mg/kg, ip) by (a) in vivo biodistribution of the uptake of [123I]Annexin V, a biomarker of the early stages of apoptosis; (b) in vitro evaluation of the apoptotic index (percentage of number of apoptotic cells versus total number of cells) in the form of DNA fragmentation, on tissue sections using in situ oligo ligation (ISOL). ISOL demonstrated a 62- and 90-fold increase of apoptotic index at 6 and 11 h after DEX administration respectively, in the outer part of the thymic lobule (cortex) and a 25- and 54-fold increases in the inner part of the thymic lobule (medulla) in the corresponding treatment groups. In the biodistribution study, [123I]Annexin V uptake was significantly increased in the thymus of rats 11 h after DEX administration (by 1.3- to 1.4-fold) and significantly decreased at the 6-h time point. We conclude that the specificity of the apoptotic signal provided by isotopic methods in vivo would always require confirmation by complementary in vitro techniques that verify the assessment of ongoing apoptosis accurately. © 2007 Springer Science+Business Media B.V.en_AU
dc.identifier.citationZavitsanou, K., Nguyen, V., Greguric, I., Chapman, J., Ballantyne, P., & Katsifis, A. (2007). Detection of apoptotic cell death in the thymus of dexamethasone treated rats using [123I] annexin V and in situ oligonucleotide ligation. Journal of Molecular Histology, 38(4), 313-319. doi:10.1007/s10735-007-9104-7en_AU
dc.identifier.govdoc8912en_AU
dc.identifier.issn1567-2379en_AU
dc.identifier.issn1567-2387en_AU
dc.identifier.issue4en_AU
dc.identifier.journaltitleJournal of Molecular Histologyen_AU
dc.identifier.pagination313-319en_AU
dc.identifier.urihttps://doi.org/10.1007/s10735-007-9104-7en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/9034en_AU
dc.identifier.volume38en_AU
dc.language.isoenen_AU
dc.publisherSpringer Natureen_AU
dc.subjectAnimal cellsen_AU
dc.subjectRatsen_AU
dc.subjectCell killingen_AU
dc.subjectOligonucleotidesen_AU
dc.subjectDexamethasoneen_AU
dc.subjectThymusen_AU
dc.subjectIn vivoen_AU
dc.subjectFragmentationen_AU
dc.subjectApoptosisen_AU
dc.titleDetection of apoptotic cell death in the thymus of dexamethasone treated rats using [123I]Annexin V and in situ oligonucleotide ligationen_AU
dc.typeJournal Articleen_AU
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