Cannabinoid administration increases 5HT1A receptor binding and mRNA expression in the hippocampus of adult but not adolescent rats

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dc.contributor.authorZavitsanou, Ken_AU
dc.contributor.authorWang, Hen_AU
dc.contributor.authorDalton, VSen_AU
dc.contributor.authorNguyen, VHen_AU
dc.date.accessioned2020-03-24T22:50:35Zen_AU
dc.date.available2020-03-24T22:50:35Zen_AU
dc.date.issued2010-08-11en_AU
dc.date.statistics2020-03-20en_AU
dc.description.abstractThe endocannabinoid and serotonin systems share a high level of overlap in terms of the physiological processes that they regulate, however, little is known about their functional interactions particularly during adolescence, a vulnerable period for both the development of psychosis and for initiation to substance use. In the present study, the effects of cannabinoid treatment on serotonin 5HT1A receptor density and mRNA expression were investigated in two age groups: Adolescent (postnatal day 35) and adult (postnatal day 70) rats were injected with the synthetic cannabinoid HU210 (25, 50 or 100 μg/kg) or vehicle for 1, 4 or 14 days and sacrificed 24 h after the last injection. 5HT1A receptor density was measured in different brain regions using [3H]8-OH-DPAT quantitative autoradiography whereas mRNA expression was measured in adjacent brain sections. Higher levels of both serotonin 5HT1A receptor binding and mRNA expression were observed in limbic regions in adolescent control animals compared to adults. 5HT1A receptor density was increased by 23% in the CA1 region of the hippocampus of adult rats treated with 100 μg/kg HU210 for 4 days compared to vehicle treated controls. The same treatment increased mRNA expression by 27% and by 14% in the CA1 region and dentate gyrus of the hippocampus respectively. 5HT1A receptor density was increased by 22% in the CA1 of adult animals treated with 50 μg HU210, by 26% in the dentate gurus of adult rats treated with 100 μg for 14 days. By contrast, 5HT1A receptor density or mRNA expression was not affected in the brain of adolescent animals in any of the brain regions examined. These results suggest that cannabinoid treatment has differential effects on serotonin-related neurochemistry in adolescent compared to adult rats. The effects in the adult brain may compromise hippocampal function and could account for the cognitive deficits seen in habitual heavy cannabis users. © 2010 IBROen_AU
dc.identifier.citationZavitsanou, K., Wang, H., Dalton, V. S., & Nguyen, V. (2010). Cannabinoid administration increases 5HT1A receptor binding and mRNA expression in the hippocampus of adult but not adolescent rats. Neuroscience, 169(1), 315-324. doi:10.1016/j.neuroscience.2010.04.005en_AU
dc.identifier.govdoc8913en_AU
dc.identifier.issn0306-4522en_AU
dc.identifier.issue1en_AU
dc.identifier.journaltitleNeuroscienceen_AU
dc.identifier.pagination315-324en_AU
dc.identifier.urihttps://doi.org/10.1016/j.neuroscience.2010.04.005en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/9229en_AU
dc.identifier.volume169en_AU
dc.language.isoenen_AU
dc.publisherElsevier B.V.en_AU
dc.subjectReceptorsen_AU
dc.subjectHippocampusen_AU
dc.subjectSerotoninen_AU
dc.subjectRatsen_AU
dc.subjectAutoradiographyen_AU
dc.subjectInteractionsen_AU
dc.titleCannabinoid administration increases 5HT1A receptor binding and mRNA expression in the hippocampus of adult but not adolescent ratsen_AU
dc.typeJournal Articleen_AU
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