Light and heavy ion beam analysis of thin biological sections

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dc.contributor.authorLee, Jen_AU
dc.contributor.authorSiegele, Ren_AU
dc.contributor.authorPastuovic, Zen_AU
dc.contributor.authorHackett, MJen_AU
dc.contributor.authorHunt, NHen_AU
dc.contributor.authorGrau, GEen_AU
dc.contributor.authorCohen, DDen_AU
dc.contributor.authorLay, PAen_AU
dc.date.accessioned2015-10-13T23:45:45Zen_AU
dc.date.available2015-10-13T23:45:45Zen_AU
dc.date.issued2013-07-01en_AU
dc.date.statistics2015-10-07en_AU
dc.description.abstractThe application of ion beam analysis (IBA) techniques to thin biological sections (ThBS) presents unique challenges in sample preparation, data acquisition and analysis. These samples are often the end product of expensive, time-consuming experiments, which involve many steps that require careful attention. Analysis via several techniques can maximise the information that is collected from these samples. Particle-induced X-ray emission (PIXE) and Rutherford backscattering (RBS) spectroscopy are two generally non-destructive IBA techniques that use the same MeV ions and can be performed simultaneously. The use of heavy ion PIXE applied to thick samples has, in the past, resulted in X-ray spectra of a poorer quality when compared to those obtained with proton beams. One of the reasons for this is the shorter probing depth of the heavy ions, which does not affect thin sample analysis. Therefore, we have investigated and compared 3-MeV proton and 36-MeV carbon ion beams on 7-μm thick mouse brain sections at the ANSTO Heavy ion microprobe (HIMP). The application of a 36-MeV C4+ ion beam for PIXE mapping of ThBS on thin Si3N4 substrate windows produced spectra of high quality that displayed close to a nine-times gain in signal yield (Z2/q) when compared to those obtained for 3-MeV protons for P, S, Cl and K but not for Fe, Cu and Zn. Image quality was overall similar; however, some elements showed better contrast and features with protons whilst others showed improved contrast with a carbon ion beam. RBS spectra with high enough counting statistics were easily obtained with 3-MeV proton beams resulting in high resolution carbon maps, however, the count rate for nitrogen and oxygen was too low. The results demonstrate that on thin samples, 36-MeV C4+ will produce good quality PIXE spectra in less time; therefore, carbon ions may be advantageous depending on which element is being studied. However, these advantages may be outweighed by the inherent disadvantages including increased ion beam damage, the necessity of very high ion energies resulting in higher neutron fields. © 2013, Elsevier B.V.en_AU
dc.identifier.citationLang Edit Lee, J., Siegele, R., Pastuovic, Z., Hackett, M. J., Hunt, N. H., Grau, G. E., Cohen, D. D., & Lay, P. A. (2013). Light and heavy ion beam analysis of thin biological sections. Paper presented at the 13th International Conference on Microprobe Technology and Applications (ICNMTA2012), 22 – 27 July 2012, Lisbon, Portugal. In Dr. T. Pinheiro, Dr. Luís C. Alves, Dr. V. Corregidor, Dr. E. Alves, Dr Adelaide P. de Jesus and Dr. Rui C. da Silva (Eds). In Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 306, 129-133. doi:10.1016/j.nimb.2012.11.045 en_AU
dc.identifier.conferenceenddate27 July 2012en_AU
dc.identifier.conferencename13th International Conference on Microprobe Technology and Applications (ICNMTA2012)en_AU
dc.identifier.conferenceplaceLisbon, Portugal.en_AU
dc.identifier.conferencestartdate22 July 2012en_AU
dc.identifier.govdoc6142en_AU
dc.identifier.issn0168-583Xen_AU
dc.identifier.journaltitleNuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atomsen_AU
dc.identifier.pagination129-133en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.nimb.2012.11.045en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/6312en_AU
dc.identifier.volume306en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectData acquisitionen_AU
dc.subjectX-ray emission analysisen_AU
dc.subjectRutherford backscattering spectroscopyen_AU
dc.subjectSelf-absorptionen_AU
dc.subjectIon beamsen_AU
dc.subjectHeavy ionsen_AU
dc.titleLight and heavy ion beam analysis of thin biological sectionsen_AU
dc.typeJournal Articleen_AU
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