Asymmetric phospholipid: lipopolysaccharide bilayers; a Gram-negative bacterial outer membrane mimic

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dc.contributor.authorClifton, LAen_AU
dc.contributor.authorSkoda, MWAen_AU
dc.contributor.authorDaulton, Een_AU
dc.contributor.authorHughes, AVen_AU
dc.contributor.authorLe Brun, APen_AU
dc.contributor.authorLakey, JHen_AU
dc.contributor.authorHolt, SAen_AU
dc.date.accessioned2015-09-28T22:31:24Zen_AU
dc.date.available2015-09-28T22:31:24Zen_AU
dc.date.issued2013-10-16en_AU
dc.date.statistics2015-09-17en_AU
dc.description.abstractThe Gram-negative bacterial outer membrane (OM) is a complex and highly asymmetric biological barrier but the small size of bacteria has hindered advances in in vivo examination of membrane dynamics. Thus, model OMs, amenable to physical study, are important sources of data. Here, we present data from asymmetric bilayers which emulate the OM and are formed by a simple two-step approach. The bilayers were deposited on an SiO2 surface by Langmuir–Blodgett deposition of phosphatidylcholine as the inner leaflet and, via Langmuir–Schaefer deposition, an outer leaflet of either Lipid A or Escherichia coli rough lipopolysaccharides (LPS). The membranes were examined using neutron reflectometry (NR) to examine the coverage and mixing of lipids between the bilayer leaflets. NR data showed that in all cases, the initial deposition asymmetry was mostly maintained for more than 16 h. This stability enabled the sizes of the headgroups and bilayer roughness of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and Lipid A, Rc-LPS and Ra-LPS to be clearly resolved. The results show that rough LPS can be manipulated like phospholipids and used to fabricate advanced asymmetric bacterial membrane models using well-known bilayer deposition techniques. Such models will enable OM dynamics and interactions to be studied under in vivo-like conditions. © 2013, The Royal Society.en_AU
dc.identifier.articlenumber20130810en_AU
dc.identifier.citationClifton, L. A., Skoda, M. W. A., Daulton, E. L., Hughes, A. V., Le Brun, A. P., Lakey, J. H., & Holt, S. A. (2013). Asymmetric phospholipid: lipopolysaccharide bilayers; a gram-negative bacterial outer membrane mimic. Journal of the Royal Society Interface, 10(89), 1-11, 20130810. doi:10.1098/rsif.2013.0810en_AU
dc.identifier.govdoc6081en_AU
dc.identifier.issn1742-5662en_AU
dc.identifier.issue89en_AU
dc.identifier.journaltitleJournal of the Royal Society Interfaceen_AU
dc.identifier.pagination1-11en_AU
dc.identifier.urihttp://dx.doi.org/10.1098/rsif.2013.0810en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/6236en_AU
dc.identifier.volume10en_AU
dc.language.isoenen_AU
dc.publisherThe Royal Societyen_AU
dc.subjectBacteriaen_AU
dc.subjectIn vivoen_AU
dc.subjectLipidsen_AU
dc.subjectMembranesen_AU
dc.subjectEscherichia colien_AU
dc.subjectLipopolysaccharidesen_AU
dc.subjectLecithinsen_AU
dc.titleAsymmetric phospholipid: lipopolysaccharide bilayers; a Gram-negative bacterial outer membrane mimicen_AU
dc.typeJournal Articleen_AU
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