Glutamate potentiates lipopolysaccharide–stimulated interleukin-10 release from neonatal rat spinal cord astrocytes

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dc.contributor.authorWerry, ELen_AU
dc.contributor.authorLiu, GJen_AU
dc.contributor.authorLovelace, MDen_AU
dc.contributor.authorNagarajah, Ren_AU
dc.contributor.authorBennett, MRen_AU
dc.date.accessioned2019-11-12T10:11:28Zen_AU
dc.date.available2019-11-12T10:11:28Zen_AU
dc.date.issued2012-04-05en_AU
dc.date.statistics2019-11-12en_AU
dc.description.abstractInterleukin-10 (IL-10) has important anti-inflammatory effects and can be protective in inflammatory conditions, such as chronic pain and infection. Exploring factors that modulate IL-10 levels may provide insight into pathomechanisms of inflammatory conditions and may provide a method of neuroprotection during these conditions. Lipopolysaccharide (LPS) stimulation of astrocytes is a source of IL-10; hence, it is of interest to investigate factors that modulate this process. Glutamate is present in increased concentrations in inflammatory conditions, and astrocytes also express glutamate receptors. The present study, therefore, investigated whether glutamate modulates LPS stimulation of IL-10 release from neonatal spinal cord astrocytes. Enzyme-linked immunosorbent assays (ELISAs) were used to quantify IL-10 release from cultured neonatal spinal cord astrocytes, and reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure IL-10 mRNA expression. Glutamate (1 mM) significantly increased LPS (1 μg/ml)-stimulated IL-10 release from astrocytes by 166% and significantly upregulated IL-10 mRNA levels. Glutamate synergistically signaled through metabotropic glutamate receptor subgroups and the phospholipase C signaling pathway. Spinal cord astrocytes may, therefore, play a larger anti-inflammatory role than first thought in situations where glutamate and a high concentration of Toll-like receptor 4 (TLR4) agonists are present. © 2012 IBRO. Published by Elsevier Ltd.en_AU
dc.identifier.citationWerry, E. L., Liu, G. J., Lovelace, M. D., Nagarajah, R., & Bennett, M. R. (2012). Glutamate potentiates lipopolysaccharide–stimulated interleukin-10 release from neonatal rat spinal cord astrocytes. Neuroscience, 207, 12-24. doi:10.1016/j.neuroscience.2012.01.039en_AU
dc.identifier.govdoc8900en_AU
dc.identifier.issn0306-4522en_AU
dc.identifier.journaltitleNeuroscienceen_AU
dc.identifier.pagination12-24en_AU
dc.identifier.urihttps://doi.org/10.1016/j.neuroscience.2012.01.039en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/9088en_AU
dc.identifier.volume207en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectCentral nervous systemen_AU
dc.subjectLipopolysaccharidesen_AU
dc.subjectGlutamic aciden_AU
dc.subjectMessenger-RNAen_AU
dc.subjectAntipyreticsen_AU
dc.subjectInflammationen_AU
dc.subjectSpinal corden_AU
dc.subjectGlutamineen_AU
dc.titleGlutamate potentiates lipopolysaccharide–stimulated interleukin-10 release from neonatal rat spinal cord astrocytesen_AU
dc.typeJournal Articleen_AU
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