SPECT imaging of glioma with radioiodinated CLINDE: evidence from a mouse GL26 glioma model

Simple item page
dc.contributor.authorTsartsalis, Sen_AU
dc.contributor.authorDumas, Nen_AU
dc.contributor.authorTournier, BBen_AU
dc.contributor.authorPham, TQen_AU
dc.contributor.authorMoulin-Sallanon, Men_AU
dc.contributor.authorGrégoire, MCen_AU
dc.contributor.authorMillet, Pen_AU
dc.contributor.authorCharnay, Yen_AU
dc.date.accessioned2020-03-24T03:30:16Zen_AU
dc.date.available2020-03-24T03:30:16Zen_AU
dc.date.issued2015-03-13en_AU
dc.description.abstractRecent research has demonstrated the potential of 18-kDa translocator protein (TSPO) to serve as a target for nuclear imaging of gliomas. The aim of this study was to evaluate SPECT imaging of GL26 mouse glioma using radioiodinated CLINDE, a TSPO-specific tracer. Methods GL26 cells, previously transfected with an enhanced green fluorescent protein (EGFP)-expressing lentivirus, were stereotactically implanted in the striatum of C57/Bl6 mice. At 4 weeks post-injection, dynamic SPECT scans with [123I]CLINDE were performed. A displacement study assessed specificity of tracer binding. SPECT images were compared to results of autoradiography, fluorescence microscopy, in situ nucleic acid hybridization, histology, and immunohistochemistry. Western blotting was performed to verify TSPO production by the tumor. Results Specific uptake of tracer by the tumor is observed with a high signal-to-noise ratio. Tracer uptake by the tumor is indeed 3.26 ± 0.32 times higher than that of the contralateral striatum, and 78% of the activity is displaceable by unlabeled CLINDE. Finally, TSPO is abundantly expressed by the GL26 cells. Conclusions The present study demonstrates the feasibility of [123I]CLINDE SPECT in translational studies and underlines its potential for clinical glioma SPECT imaging. © 2015, Springer Natureen_AU
dc.identifier.citationTsartsalis, S., Dumas, N., Tournier, B. B., Pham, T., Moulin-Sallanon, M., Grégoire, M. C., Charnay. Y & & Millet, P. (2015). SPECT imaging of glioma with radioiodinated CLINDE: evidence from a mouse GL26 glioma model. EJNMMI Research, 5(1), 9. doi:10.1186/s13550-015-0092-4en_AU
dc.identifier.govdoc8890en_AU
dc.identifier.issn2191-219Xen_AU
dc.identifier.issue1en_AU
dc.identifier.journaltitleEJNMMI Researchen_AU
dc.identifier.pagination9en_AU
dc.identifier.urihttps://doi.org/10.1186/s13550-015-0092-4en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/9220en_AU
dc.identifier.volume5en_AU
dc.language.isoenen_AU
dc.publisherSpringer Natureen_AU
dc.subjectSingle photon emission computed tomographyen_AU
dc.subjectGliomasen_AU
dc.subjectAutoradiographyen_AU
dc.subjectTracer techniquesen_AU
dc.subjectHybridizationen_AU
dc.subjectHistologyen_AU
dc.subjectFluorescenceen_AU
dc.subjectMicroscopyen_AU
dc.titleSPECT imaging of glioma with radioiodinated CLINDE: evidence from a mouse GL26 glioma modelen_AU
dc.typeJournal Articleen_AU
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s13550-015-0092-4.pdf
Size:
1.53 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal Articles