Bardoxolone methyl prevents fat deposition and inflammation in brown adipose tissue and enhances sympathetic activity in mice fed a high-fat diet

Simple item page
dc.contributor.authorDinh, CHLen_AU
dc.contributor.authorSzabo, Aen_AU
dc.contributor.authorYu, YHen_AU
dc.contributor.authorCamer, Den_AU
dc.contributor.authorZhang, QSen_AU
dc.contributor.authorWang, HQen_AU
dc.contributor.authorHuang, XFen_AU
dc.date.accessioned2018-09-14T05:36:41Zen_AU
dc.date.available2018-09-14T05:36:41Zen_AU
dc.date.issued2015-06-09en_AU
dc.date.statistics2018-09-10en_AU
dc.descriptionThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). Copy acquired from MDPI: http://www.mdpi.com/2072-6643/7/6/4705.en_AU
dc.description.abstractObesity results in changes in brown adipose tissue (BAT) morphology, leading to fat deposition, inflammation, and alterations in sympathetic nerve activity. Bardoxolone methyl (BARD) has been extensively studied for the treatment of chronic diseases. We present for the first time the effects of oral BARD treatment on BAT morphology and associated changes in the brainstem. Three groups (n = 7) of C57BL/6J mice were fed either a high-fat diet (HFD), a high-fat diet supplemented with BARD (HFD/BARD), or a low-fat diet (LFD) for 21 weeks. BARD was administered daily in drinking water. Interscapular BAT, and ventrolateral medulla (VLM) and dorsal vagal complex (DVC) in the brainstem, were collected for analysis by histology, immunohistochemistry and Western blot. BARD prevented fat deposition in BAT, demonstrated by the decreased accumulation of lipid droplets. When administered BARD, HFD mice had lower numbers of F4/80 and CD11c macrophages in the BAT with an increased proportion of CD206 macrophages, suggesting an anti-inflammatory effect. BARD increased phosphorylation of tyrosine hydroxylase in BAT and VLM. In the VLM, BARD increased energy expenditure proteins, including beta 3-adrenergic receptor (β3-AR) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α). Overall, oral BARD prevented fat deposition and inflammation in BAT, and stimulated sympathetic nerve activity. © 2015 by the authors; licensee MDPI, Basel, Switzerlanden_AU
dc.identifier.citationDinh, C. H. L., Szabo, A., Yu, Y., Camer, D., Zhang, Q., Wang, H., & Huang, X. F. (2015). Bardoxolone methyl prevents fat deposition and inflammation in brown adipose tissue and enhances sympathetic activity in mice fed a high-fat diet. Nutrients, 7(6), 4705-4723. doi:10.3390/nu7064705en_AU
dc.identifier.govdoc8723en_AU
dc.identifier.issn2072-6643en_AU
dc.identifier.issue6en_AU
dc.identifier.journaltitleNutrientsen_AU
dc.identifier.pagination4705-4723en_AU
dc.identifier.urihttps://doi.org/10.3390/nu7064705en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/8987en_AU
dc.identifier.volume7en_AU
dc.language.isoenen_AU
dc.publisherMDPIen_AU
dc.subjectMetabolic diseasesen_AU
dc.subjectAdipose tissueen_AU
dc.subjectFatsen_AU
dc.subjectDieten_AU
dc.subjectKidneysen_AU
dc.subjectDiseasesen_AU
dc.subjectInflammationen_AU
dc.titleBardoxolone methyl prevents fat deposition and inflammation in brown adipose tissue and enhances sympathetic activity in mice fed a high-fat dieten_AU
dc.typeJournal Articleen_AU
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
nutrients-07-04705.pdf
Size:
3.5 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal Articles