Picosecond dynamics in haemoglobin from different species: a quasielastic neutron scattering study

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dc.contributor.authorStadler, AMen_AU
dc.contributor.authorGarvey, CJen_AU
dc.contributor.authorEmbs, JPen_AU
dc.contributor.authorKoza, MMen_AU
dc.contributor.authorUnruh, Ten_AU
dc.contributor.authorArtmann, GMen_AU
dc.contributor.authorZaccai, Gen_AU
dc.date.accessioned2016-10-30T23:22:58Zen_AU
dc.date.available2016-10-30T23:22:58Zen_AU
dc.date.issued2014-10en_AU
dc.date.statistics2016-10-31en_AU
dc.description.abstractBackground Dynamics in haemoglobin from platypus (Ornithorhynchus anatinus), chicken (Gallus gallus domesticus) and saltwater crocodile (Crocodylus porosus) were measured to investigate response of conformational motions on the picosecond time scale to naturally occurring variations in the amino acid sequence of structurally identical proteins. Methods Protein dynamics was measured using incoherent quasielastic neutron scattering. The quasielastic broadening was interpreted first with a simple single Lorentzian approach and then by using the Kneller–Volino Brownian dynamics model. Results Mean square displacements of conformational motions, diffusion coefficients of internal dynamics and residence times for jump-diffusion between sites and corresponding effective force constants (resilience) and activation energies were determined from the data. Conclusions Modifications of the physicochemical properties caused by mutations of the amino acids were found to have a significant impact on protein dynamics. Activation energies of local side chain dynamics were found to be similar between the different proteins being close to the energy, which is required for the rupture of single hydrogen bond in a protein. General significance The measured dynamic quantities showed significant and systematic variations between the investigated species, suggesting that they are the signature of an evolutionary adaptation process stimulated by the different physiological environments of the respective protein. © 2014,Elsevier B.V.en_AU
dc.identifier.citationStadler, A. M., Garvey, C. J., Embs, J. P., Koza, M. M., Unruh, T., Artmann, G., & Zaccai, G. (2014). Picosecond dynamics in haemoglobin from different species: a quasielastic neutron scattering study. Biochimica et Biophysica Acta (BBA) - General Subjects, 1840(10), 2989-2999. doi:10.1016/j.bbagen.2014.06.007en_AU
dc.identifier.govdoc7540en_AU
dc.identifier.issn0304-4165en_AU
dc.identifier.journaltitleBiochimica et Biophysica Acta (BBA) - General Subjectsen_AU
dc.identifier.pagination2989-2999en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.bbagen.2014.06.007en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/7978en_AU
dc.identifier.volume1840en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectBody temperatureen_AU
dc.subjectProteinsen_AU
dc.subjectAmino acidsen_AU
dc.subjectHydrogenen_AU
dc.subjectBlood cellsen_AU
dc.subjectOxygenen_AU
dc.titlePicosecond dynamics in haemoglobin from different species: a quasielastic neutron scattering studyen_AU
dc.typeJournal Articleen_AU
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