Neutron reflectometry studies define prion protein N-terminal peptide membrane binding

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dc.contributor.authorLe Brun, APen_AU
dc.contributor.authorHaigh, CLen_AU
dc.contributor.authorDrew, SCen_AU
dc.contributor.authorJames, Men_AU
dc.contributor.authorBoland, MPen_AU
dc.contributor.authorCollins, SJen_AU
dc.date.accessioned2016-09-14T01:55:11Zen_AU
dc.date.available2016-09-14T01:55:11Zen_AU
dc.date.issued2014-11-18en_AU
dc.date.statistics2016-09-14en_AU
dc.description.abstractThe prion protein (PrP), widely recognized to misfold into the causative agent of the transmissible spongiform encephalopathies, has previously been shown to bind to lipid membranes with binding influenced by both membrane composition and pH. Aside from the misfolding events associated with prion pathogenesis, PrP can undergo various posttranslational modifications, including internal cleavage events. Alpha- and beta-cleavage of PrP produces two N-terminal fragments, N1 and N2, respectively, which interact specifically with negatively charged phospholipids at low pH. Our previous work probing N1 and N2 interactions with supported bilayers raised the possibility that the peptides could insert deeply with minimal disruption. In the current study we aimed to refine the binding parameters of these peptides with lipid bilayers. To this end, we used neutron reflectometry to define the structural details of this interaction in combination with quartz crystal microbalance interrogation. Neutron reflectometry confirmed that peptides equivalent to N1 and N2 insert into the interstitial space between the phospholipid headgroups but do not penetrate into the acyl tail region. In accord with our previous studies, interaction was stronger for the N1 fragment than for the N2, with more peptide bound per lipid. Neutron reflectometry analysis also detected lengthening of the lipid acyl tails, with a concurrent decrease in lipid area. This was most evident for the N1 peptide and suggests an induction of increased lipid order in the absence of phase transition. These observations stand in clear contrast to the findings of analogous studies of Ab and α-synuclein and thereby support the possibility of a functional role for such N-terminal fragment-membrane interactions. © 2014,Biophysical Society.en_AU
dc.identifier.citationLe Brun, A. P., Haigh, C. L., Drew, S. C., James, M., Boland, M. P., & Collins, S. J. (2014) Neutron reflectometry studies define prion protein N-terminal peptide membrane binding. Biophysical Journal, 107(10), 2313-2324. doi: 10.1016/j.bpj.2014.09.027en_AU
dc.identifier.govdoc7085en_AU
dc.identifier.issn0006-3495en_AU
dc.identifier.issue10en_AU
dc.identifier.journaltitleBiophysical Journalen_AU
dc.identifier.pagination2313-2324en_AU
dc.identifier.urihttps://doi.org/10.1016/j.bpj.2014.09.027en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/7501en_AU
dc.identifier.volume107en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectProteinsen_AU
dc.subjectLipidsen_AU
dc.subjectMembranesen_AU
dc.subjectPathogenesisen_AU
dc.subjectPhospholipidsen_AU
dc.subjectNeutron reflectorsen_AU
dc.titleNeutron reflectometry studies define prion protein N-terminal peptide membrane bindingen_AU
dc.typeJournal Articleen_AU
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