High coverage fluid-phase floating lipid bilayers supported by ω-thiolipid self-assembled monolayers

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dc.contributor.authorHughes, AVen_AU
dc.contributor.authorHolt, SAen_AU
dc.contributor.authorDaulton, Een_AU
dc.contributor.authorSoliakov, Aen_AU
dc.contributor.authorCharlton, TRen_AU
dc.contributor.authorRoser, SJen_AU
dc.contributor.authorLakey, JHen_AU
dc.date.accessioned2016-08-26T05:47:00Zen_AU
dc.date.available2014-09-06T05:47:00Zen_AU
dc.date.issued2014-09-06en_AU
dc.date.statistics2016-08-26en_AU
dc.description.abstractLarge area lipid bilayers, on solid surfaces, are useful in physical studies of biological membranes. It is advantageous to minimize the interactions of these bilayers with the substrate and this can be achieved via the formation of a floating supported bilayer (FSB) upon either a surface bound phospholipid bilayer or monolayer. The FSB's independence is enabled by the continuous water layer (greater than 15 Å) that remains between the two. However, previous FSBs have had limited stability and low density. Here, we demonstrate by surface plasmon resonance and neutron reflectivity, the formation of a complete self-assembled monolayer (SAM) on gold surfaces by a synthetic phosphatidylcholine bearing a thiol group at the end of one fatty acyl chain. Furthermore, a very dense FSB (more than 96%) of saturated phosphatidylcholine can be formed on this SAM by sequential Langmuir–Blodgett and Langmuir–Schaefer procedures. Neutron reflectivity used both isotopic and magnetic contrast to enhance the accuracy of the data fits. This system offers the means to study transmembrane proteins, membrane potential effects (using the gold as an electrode) and even model bacterial outer membranes. Using unsaturated phosphatidylcholines, which have previously failed to form stable FSBs, we achieved a coverage of 73%. © 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.en_AU
dc.identifier.articlenumber20140447en_AU
dc.identifier.citationHughes, A. V., Holt, S. A., Daulton, E., Soliakov, A., Charlton, T. R., Roser, S. J., & Lakey, J. H. (2014). High coverage fluid-phase floating lipid bilayers supported by ω-thiolipid self-assembled monolayers. Journal of The Royal Society Interface, 11(98), 20140447. doi:10.1098/rsif.2014.0447en_AU
dc.identifier.govdoc7027en_AU
dc.identifier.issn1742-5662en_AU
dc.identifier.journaltitleJournal of The Royal Society Interfaceen_AU
dc.identifier.urihttp://dx.doi.org/10.1098/rsif.2014.0447en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/7384en_AU
dc.identifier.volume11en_AU
dc.language.isoenen_AU
dc.publisherThe Royal Society Publishingen_AU
dc.subjectBiochemistryen_AU
dc.subjectBiophysicsen_AU
dc.subjectLipidsen_AU
dc.subjectProteinsen_AU
dc.subjectGolden_AU
dc.subjectNeutron reflectorsen_AU
dc.subjectMembranesen_AU
dc.titleHigh coverage fluid-phase floating lipid bilayers supported by ω-thiolipid self-assembled monolayersen_AU
dc.typeJournal Articleen_AU
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