BioPEGylation of polyhydroxybutyrate promotes nerve cell health and migration

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dc.contributor.authorChan, RTHen_AU
dc.contributor.authorRussell, RAen_AU
dc.contributor.authorMarçal, Hen_AU
dc.contributor.authorLee, THen_AU
dc.contributor.authorHolden, PJen_AU
dc.contributor.authorFoster, LJRen_AU
dc.date.accessioned2016-06-22T05:57:50Zen_AU
dc.date.available2016-06-22T05:57:50Zen_AU
dc.date.issued2013-12-03en_AU
dc.date.statistics2016-06-22en_AU
dc.description.abstractThis study reports on the superior suitability of Polyhydroxybutyrate-polyethylene glycol hybrid polymers biosynthesised by Cupriavidus necator over PHB as biomaterials for tissue engineering. Incorporation of PEG106 (DEG) during PHB biosynthesis reduced crystallinity, molecular weight, and hydrophobicity while improving mechanical properties. In vitro olfactory ensheathing cell (OEC) proliferation was enhanced by cultivation on PHB-b-DEG films. Cultivation on PHB and PHB-b-DEG films showed no cytotoxic responses and cell viability and membrane integrity was sustained. PHB-b-DEG films promoted OECs entering into the DNA replication (S) phase and mitotic (G2-M) phase during the cell growth cycle and apoptosis was low. This study also confirmed an association between the level of neurite-outgrowth inhibitory protein (Nogo) and receptor pair Ig-like receptor B (PirB) expression and cell proliferation, both being down-regulated in cells grown on hybrid films when compared with PHB and asynchronous growth. Thus, DEG-terminated PHB-based biomaterials have great potential as biological scaffolds supporting nerve repair. © 2013 American Chemical Society.en_AU
dc.identifier.citationChan, R. T. H., Russell, R. A., Marçal, H., Lee, T. H., Holden, P. J., & Foster, L. J. R. (2014). BioPEGylation of polyhydroxybutyrate promotes nerve cell health and migration. Biomacromolecules, 15(1), 339-349. doi:10.1021/bm401572aen_AU
dc.identifier.govdoc6737en_AU
dc.identifier.issn1525-7797en_AU
dc.identifier.issue1en_AU
dc.identifier.journaltitleBiomacromoleculesen_AU
dc.identifier.pagination339-349en_AU
dc.identifier.urihttp://dx.doi.org/10.1021/bm401572aen_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/7058en_AU
dc.identifier.volume15en_AU
dc.language.isoenen_AU
dc.publisherACS Publicationsen_AU
dc.subjectPolyethylenesen_AU
dc.subjectGlycolsen_AU
dc.subjectBiosynthesisen_AU
dc.subjectMolecular structureen_AU
dc.subjectCrystallizationen_AU
dc.subjectProteinsen_AU
dc.titleBioPEGylation of polyhydroxybutyrate promotes nerve cell health and migrationen_AU
dc.typeJournal Articleen_AU
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