The role of additives in moderating the influence of Fe(III) and Cu(II) on the radiochemical yield of [68Ga(DOTATATE)]

Simple item page
dc.contributor.authorOehlke, Een_AU
dc.contributor.authorLengkeek, ALen_AU
dc.contributor.authorLe, VSen_AU
dc.contributor.authorPellegrini, PAen_AU
dc.contributor.authorGreguric, Ien_AU
dc.contributor.authorWeiner, Ren_AU
dc.date.accessioned2018-09-19T02:06:24Zen_AU
dc.date.available2018-09-19T02:06:24Zen_AU
dc.date.issued2016-01en_AU
dc.date.statistics2018-09-18en_AU
dc.description.abstract68Ga(DOTATATE)] has demonstrated its clinical usefulness. Both Fe3+ and Cu2+, potential contaminants in Gallium-68 generator eluent, substantially reduce the radiochemical (RC) yield of [68Ga(DOTATATE)] if the metal/ligand ratio of 1:1 is exceeded. A variety of compounds were examined for their potential ability to reduce this effect. Most had no effect on RC yield. However, addition of phosphate diminished the influence of Fe3+ by likely forming an insoluble iron salt. Addition of ascorbic acid reduced Cu2+ and Fe3+ to Cu+ and Fe2+ respectively, both of which have limited impact on RC yields. At low ligand amounts (5 nmol DOTATATE), the addition of 30 nmol phosphate (0.19 mM) increased the tolerance of Fe3+ from 4 nmol to 10 nmol (0.06 mM), while the addition of ascorbic acid allowed high RC yields (>95%) in the presence of 40 nmol Fe3+ (0.25 mM) and 100 nmol Cu2+ (0.63 mM). The effect of ascorbic acid was highly pH-dependant, and gave optimal results at pH 3. © 2015 Elsevier B.V.en_AU
dc.identifier.citationOehlke, E., Lengkeek, N. A., Pellegrini, P. A., Greguric, I., & Weiner, R. (2016). The role of additives in moderating the influence of Fe(III) and Cu(II) on the radiochemical yield of [68Ga(DOTATATE)]. Applied Radiation and Isotopes, 107, 13-16. doi:10.1016/j.apradiso.2015.09.008en_AU
dc.identifier.govdoc8828en_AU
dc.identifier.issn0969-8043en_AU
dc.identifier.journaltitleApplied Radiation and Isotopesen_AU
dc.identifier.pagination13-17en_AU
dc.identifier.urihttps://doi.org/10.1016/j.apradiso.2015.09.008en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/9020en_AU
dc.identifier.volume107en_AU
dc.language.isoenen_AU
dc.publisherElsevier B.V.en_AU
dc.subjectAscorbic aciden_AU
dc.subjectPhosphatesen_AU
dc.subjectAdditivesen_AU
dc.subjectPositron computed tomographyen_AU
dc.subjectBiological pathwaysen_AU
dc.subjectContaminationen_AU
dc.subjectLigandsen_AU
dc.subjectSaltsen_AU
dc.titleThe role of additives in moderating the influence of Fe(III) and Cu(II) on the radiochemical yield of [68Ga(DOTATATE)]en_AU
dc.typeJournal Articleen_AU
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal Articles