Peptide modification of purified gellan gum

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dc.contributor.authorFerris, CJen_AU
dc.contributor.authorStevens, LRen_AU
dc.contributor.authorGilmore, KJen_AU
dc.contributor.authorMume, Een_AU
dc.contributor.authorGreguric, IDen_AU
dc.contributor.authorKirchmajer, DMen_AU
dc.contributor.authorWallace, GGen_AU
dc.contributor.authorin het Panhuis, Men_AU
dc.date.accessioned2018-09-16T23:39:30Zen_AU
dc.date.available2018-09-16T23:39:30Zen_AU
dc.date.issued2014-12-10en_AU
dc.date.statistics2018-09-16en_AU
dc.description.abstractAbstractGellan gum (GG) is an anionic polysaccharide with potential as a biopolymer for additive manufacturing (3D-bioprinting) and tissue engineering. Previous studies have shown GG to be highly cytocompatible, but lacking specific attachment sites required for anchorage-dependent cells. In this work, we modify purified-GG polymer with a short peptide containing the arginine-glycine-aspartic acid (RGD) sequence that is known to enhance integrin-mediated cell attachment. Radiolabelling of the peptide was used in optimisation of the conjugation procedure to achieve an overall efficiency of 40%. The purification of divalent cations from commercial GG samples was found to be critical for successful conjugation. Rheological studies revealed that the peptide coupling did not prevent gelation behaviour. C2C12 cells showed improved attachment on the surface of and encapsulated within RGD-GG hydrogels, differentiating to multinucleated myofibers after 5–7 days. PC12 cells showed minimal interactions with both GG and RGD-GG, with formation of cell clusters and impedance of terminal differentiation and neurite extension. © 2015 Royal Society of Chemistryen_AU
dc.identifier.citationFerris, C. J., Stevens, L. R., Gilmore, K. J., Mume, E., Greguric, I., Kirchmajer, D. M., Wallace, G. G., & in het Panhuis, M. (2015). Peptide modification of purified gellan gum. Journal of Materials Chemistry B, 3(6), 1106-1115. doi:10.1039/C4TB01727Gen_AU
dc.identifier.govdoc8748en_AU
dc.identifier.issn2050-7518en_AU
dc.identifier.issue6en_AU
dc.identifier.journaltitleJournal of Materials Chemistry Ben_AU
dc.identifier.pagination1106-1115en_AU
dc.identifier.urihttps://doi.org/10.1039/c4tb01727gen_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/9000en_AU
dc.identifier.volume3en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectGumsen_AU
dc.subjectPeptidesen_AU
dc.subjectPolysaccharidesen_AU
dc.subjectArginineen_AU
dc.subjectAspartic aciden_AU
dc.subjectGelationen_AU
dc.subjectHydrogelsen_AU
dc.subjectPolymersen_AU
dc.titlePeptide modification of purified gellan gumen_AU
dc.typeJournal Articleen_AU
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