Preclinical characterization of 18FD-FPHCys, a new amino acid-based PET tracer

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dc.contributor.authorDenoyer, Den_AU
dc.contributor.authorKirby, Len_AU
dc.contributor.authorWaldeck, Ken_AU
dc.contributor.authorRoselt, Pen_AU
dc.contributor.authorNeels, OCen_AU
dc.contributor.authorBourdier, Ten_AU
dc.contributor.authorShepherd, Ren_AU
dc.contributor.authorKatsifis, Aen_AU
dc.contributor.authorHicks, RJen_AU
dc.date.accessioned2014-08-20T02:02:53Zen_AU
dc.date.available2014-08-20T02:02:53Zen_AU
dc.date.issued2012-04-01en_AU
dc.date.statistics2012-08-20en_AU
dc.description.abstractThe imaging potential of a new F-18-labelled methionine derivative, S-(3-[F-18]fluoropropyl)-d-homocysteine (F-18-D-FPHCys), and its selectivity for amino acid transporter subtypes were investigated in vitro and by imaging of human tumour xenografts. Expression of members of the system L (LAT isoforms 1-4 and 4F2hc) and ASCT (ASCT isoforms 1 and 2) amino acid transporter subclasses were assessed by quantitative real-time PCR in four human tumour models, including A431 squamous cell carcinoma, PC3 prostate cancer, and Colo 205 and HT-29 colorectal cancer lines. The first investigations for the characterization of F-18-D-FPHCys were in vitro uptake studies by comparing it with [1-C-14]-l-methionine (C-14-MET) and in vivo by PET imaging. In addition, the specific involvement of LAT1 transporters in F-18-D-FPHCys accumulation was tested by silencing LAT1 mRNA transcription with siRNAs. To determine the proliferative activity in tumour xenografts ex vivo, Ki-67 staining was used as a biomarker. A431 cells showed the highest F-18-D-FPHCys uptake in vitro and in vivo followed by Colo 205, PC3 and HT-29. A similar pattern of retention was observed with C-14-MET. F-18-D-FPHCys retention was strongly correlated with LAT1 expression both in vitro (R (2) = 0.85) and in vivo (R (2) = 0.99). Downregulation of LAT1 by siRNA inhibited F-18-D-FPHCys uptake, demonstrating a clear dependence on this transporter for tumour uptake. Furthermore, F-18-D-FPHCys accumulation mirrored cellular proliferation. The favourable properties of F-18-D-FPHCys make this tracer a promising imaging probe for detection of tumours as well as for the noninvasive evaluation and monitoring of tumour growth.© 2012, Springer.en_AU
dc.identifier.citationDenoyer, D., Kirby, L., Waldeck, K., Roselt, P., Neels, O.C., Bourdier, T., Shepherd, R., Katsifis, A., & Hicks, R.J. (2012). Preclinical characterization of f-18-d-fphcys, a new amino acid-based pet tracer. European Journal of Nuclear Medicine and Molecular Imaging, 39(4), 703-712. doi:10.1007/s00259-011-2017-4en_AU
dc.identifier.govdoc5705en_AU
dc.identifier.issn1619-7070en_AU
dc.identifier.issue4en_AU
dc.identifier.journaltitleEuropean Journal of Nuclear Medicine and Molecular Imagingen_AU
dc.identifier.pagination703-712en_AU
dc.identifier.urihttps://doi.org/10.1007/s00259-011-2017-4en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/5796en_AU
dc.identifier.volume39en_AU
dc.language.isoenen_AU
dc.publisherSpringeren_AU
dc.subjectTyrosineen_AU
dc.subjectMethionineen_AU
dc.subjectTomographyen_AU
dc.subjectIn vitroen_AU
dc.subjectNeoplasmsen_AU
dc.subjectHomocysteineen_AU
dc.titlePreclinical characterization of 18FD-FPHCys, a new amino acid-based PET traceren_AU
dc.typeJournal Articleen_AU
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