Phospholipid membrane protection by sugar molecules during dehydration-insights into molecular mechanisms using scattering techniques

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dc.contributor.authorGarvey, CJen_AU
dc.contributor.authorLenné, Ten_AU
dc.contributor.authorKoster, KLen_AU
dc.contributor.authorKent, Ben_AU
dc.contributor.authorBryant, Gen_AU
dc.date.accessioned2014-01-20T05:40:58Zen_AU
dc.date.available2014-01-20T05:40:58Zen_AU
dc.date.issued2013-04-01en_AU
dc.date.statistics2014-01-20en_AU
dc.description.abstractScattering techniques have played a key role in our understanding of the structure and function of phospholipid membranes. These techniques have been applied widely to study how different molecules (e. g., cholesterol) can affect phospholipid membrane structure. However, there has been much less attention paid to the effects of molecules that remain in the aqueous phase. One important example is the role played by small solutes, particularly sugars, in protecting phospholipid membranes during drying or slow freezing. In this paper, we present new results and a general methodology, which illustrate how contrast variation small angle neutron scattering (SANS) and synchrotron-based X-ray scattering (small angle (SAXS) and wide angle (WAXS)) can be used to quantitatively understand the interactions between solutes and phospholipids. Specifically, we show the assignment of lipid phases with synchrotron SAXS and explain how SANS reveals the exclusion of sugars from the aqueous region in the particular example of hexagonal II phases formed by phospholipids.© 2011, MDPI Publishing. © 2013, MDPI Publishingen_AU
dc.identifier.citationGarvey, C. J., Lenné, T., Koster, K. L., Kent, B., & Bryant, G. J. (2013). Phospholipid membrane protection by sugar molecules during dehydration-insights into molecular mechanisms using scattering techniques. International Journal of Molecular Sciences, 14 (4), 8148-8163. doi:doi.org/10.3390/ijms14048148en_AU
dc.identifier.govdoc5154en_AU
dc.identifier.issn1422-0067en_AU
dc.identifier.issue4en_AU
dc.identifier.journaltitleInternational Journal of Molecular Sciencesen_AU
dc.identifier.pagination8148-8163en_AU
dc.identifier.urihttp://dx.doi.org/10.3390/ijms14048148en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/5156en_AU
dc.identifier.volume14en_AU
dc.language.isoenen_AU
dc.publisherMDPI AGen_AU
dc.subjectScatteringen_AU
dc.subjectNeutronsen_AU
dc.subjectInteractionsen_AU
dc.subjectPhospholipidsen_AU
dc.subjectMembranesen_AU
dc.subjectCholesterolen_AU
dc.titlePhospholipid membrane protection by sugar molecules during dehydration-insights into molecular mechanisms using scattering techniquesen_AU
dc.typeJournal Articleen_AU
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