Poly(ethylene glycol)-modulated cellular biocompatibility of polyhydroxyalkanoate films

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dc.contributor.authorChan, RTHen_AU
dc.contributor.authorMarçal, Hen_AU
dc.contributor.authorAhmed, Ten_AU
dc.contributor.authorRussell, RAen_AU
dc.contributor.authorHolden, PJen_AU
dc.contributor.authorFoster, LJRen_AU
dc.date.accessioned2013-11-12T05:12:18Zen_AU
dc.date.available2013-11-12T05:12:18Zen_AU
dc.date.issued2013-06-01en_AU
dc.date.statistics2013-11-12en_AU
dc.description.abstractPolyhydroxybutyrate (PHB) and its copolymer with hydroxyvalerate, P(HB-co-HV), are widely used biomaterials. In this study, improvements of their biological properties of degradability and compatibility were achieved by blending with low-molecular-weight poly(ethylene glycol) (PEG106) approved for medical use. Surface morphology and chemistry are known to support cell attachment. Attachment and proliferation of neural olfactory ensheathing cells increased by 17.0 and 32.2% for PHB and P(HB-co-HV) composite films. Cell attachment was facilitated by increases in surface hydrophilicity, water contact angles decreased by 26 ± 2° and water uptake increased by 23.3% depending upon biopolymer and PEG loading. Cells maintained high viability (>95%) on the composite films with no evidence of cytotoxic effects. Assays of mitochondrial function and cell leakage showed improved cell health as a consequence of PEG loading. The PEG component was readily solubilised from composite films, allowing control of degradation profiles in the cell growth medium. Promotion of biopolymer compatibility and degradability was not at the expense of material properties, with the extension to break of the composites increasing by 5.83 ± 1.06%. Similarly, crystallinity decreased by 36%. The results show that blending of common polyhydroxyalkanoate biomaterials with low-molecular-weight PEG can be used to promote biocompatibility and manipulate physiochemical and material properties as well as degradation. © 2013, Wiley-Blackwell.en_AU
dc.identifier.citationChan, R. T. H., Marçal, H., Ahmed, T., Russell, R. A., Holden, P. J., & Foster, L. J. R. (2013). Poly(ethylene glycol)-modulated cellular biocompatibility of polyhydroxyalkanoate films. Polymer International, 62 (6), 884-892. doi:10.1002/pi.4451en_AU
dc.identifier.govdoc5121en_AU
dc.identifier.issn0959-8103en_AU
dc.identifier.issue6en_AU
dc.identifier.journaltitlePolymer Internationalen_AU
dc.identifier.pagination884-892en_AU
dc.identifier.urihttp://dx.doi.org/10.1002/pi.4451en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/4940en_AU
dc.identifier.volume62en_AU
dc.language.isoenen_AU
dc.publisherWiley-Blackwellen_AU
dc.subjectFilmsen_AU
dc.subjectWateren_AU
dc.subjectDecompositionen_AU
dc.subjectProliferationen_AU
dc.subjectChemistryen_AU
dc.subjectMixingen_AU
dc.titlePoly(ethylene glycol)-modulated cellular biocompatibility of polyhydroxyalkanoate filmsen_AU
dc.typeJournal Articleen_AU
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