Structural characterization of a model gram-negative bacterial surface using lipopolysaccharides from rough strains of escherichia coli

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dc.contributor.authorLe Brun, APen_AU
dc.contributor.authorClifton, LAen_AU
dc.contributor.authorHalbert, CEen_AU
dc.contributor.authorLin, Ben_AU
dc.contributor.authorMeron, Men_AU
dc.contributor.authorHolden, PJen_AU
dc.contributor.authorLakey, JHen_AU
dc.contributor.authorHolt, SAen_AU
dc.date.accessioned2013-10-10T04:03:33Zen_AU
dc.date.available2013-10-10T04:03:33Zen_AU
dc.date.issued2013-06-01en_AU
dc.date.statistics2013-10-10en_AU
dc.description.abstractLipopolysaccharides (LPS) make up approximately 75% of the Gram-negative bacterial outer membrane (OM) surface, but because of the complexity of the molecule, there are very few model OMs that include LPS. The LPS molecule consists of lipid A, which anchors the LPS within the OM, a core polysaccharide region, and a variable O-antigen polysaccharide chain. In this work we used RcLPS (consisting of lipid A plus the first seven sugars of the core polysaccharide) from a rough strain of Escherichia coli to form stable monolayers of LPS at the air?liquid interface. The vertical structure RcLPS monolayers were characterized using neutron and X-ray reflectometry, while the lateral structure was investigated using grazing incidence X-ray diffraction and Brewster angle microscopy. It was found that RcLPS monolayers at surface pressures of 20 mN m(-1) and above are resolved as hydrocarbon tails, an inner headgroup, and an outer headgroup of polysaccharide with increasing solvation from tails to outer headgroups. The lateral organization of the hydrocarbon lipid chains displays an oblique hexagonal unit cell at all surface pressures, with only the chain tilt angle changing with surface pressure. This is in contrast to lipid A, which displays hexagonal or, above 20 mN m(-1), distorted hexagonal packing. This work provides the first complete structural analysis of a realistic E. coli OM surface model. © 2013, American Chemical Society.en_AU
dc.identifier.citationLe Brun, A. P., Clifton, L. A., Halbert, C. E., Lin, B., Meron, M., Holden, P. J., Lakey, J. H., & Holt, S. A. (2013). Structural characterization of a model gram-negative bacterial surface using lipopolysaccharides from rough strains of escherichia coli. Biomacromolecules, 14 (6), 2014-2022. doi:10.1021/bm400356men_AU
dc.identifier.govdoc5096en_AU
dc.identifier.issn1525-7797en_AU
dc.identifier.issue6en_AU
dc.identifier.journaltitleBiomacromoleculesen_AU
dc.identifier.pagination2014-2022en_AU
dc.identifier.urihttp://dx.doi.org/10.1021/bm400356men_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/4782en_AU
dc.identifier.volume14en_AU
dc.language.isoenen_AU
dc.publisherAmerican Chemical Societyen_AU
dc.subjectX-ray diffractionen_AU
dc.subjectMicroscopyen_AU
dc.subjectLipopolysaccharidesen_AU
dc.subjectTransition elementsen_AU
dc.subjectLipidsen_AU
dc.subjectCarbohydratesen_AU
dc.subjectHydrocarbonsen_AU
dc.titleStructural characterization of a model gram-negative bacterial surface using lipopolysaccharides from rough strains of escherichia colien_AU
dc.typeJournal Articleen_AU
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