Calcium mediated interaction of calf-thymus DNA with monolayers of distearoylphosphatidylcholine: a neutron and X-ray reflectivity study

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dc.contributor.authorDabkowska, APen_AU
dc.contributor.authorTalbot, JPen_AU
dc.contributor.authorCavalcanti, Len_AU
dc.contributor.authorWebster, JRPen_AU
dc.contributor.authorNelson, Aen_AU
dc.contributor.authorBarlow, DJen_AU
dc.contributor.authorFragneto, Gen_AU
dc.contributor.authorLawrence, MJen_AU
dc.date.accessioned2014-07-03T01:43:44Zen_AU
dc.date.available2014-07-03T01:43:44Zen_AU
dc.date.issued2013-01-01en_AU
dc.date.statistics2014-07-03en_AU
dc.description.abstractX-ray and neutron reflection studies, the latter in conjunction with contrast variation, have been combined to study the interaction of calf thymus DNA (ctDNA) with monolayers of distearoylphosphatidylcholine (DSPC) in the presence of 20 mM Ca2+ ions, at the air-liquid interface as a function of surface pressure (10, 20, 30 and 40 mN m-1). Analysis of the X-ray and neutron reflection data showed that, regardless of the surface pressure of the monolayer, a layer of ctDNA was present below the DSPC lipid head groups and that this ctDNA-containing layer (thickness [similar]12.5 to 15 A) was separated from the DSPC head groups by a layer of water of [similar]9 A thickness. The thickness of the ctDNA-containing layer was thinner than that reported for monolayers of cationic lipid at the air-water interface (18-25 A) although in these monolayers no water layer separating the lipid head groups from the layer containing ctDNA has been reported. At all surface pressures the amount of ctDNA present in the layer was in the range 30-40% by volume. As no significant re-arrangement of the DSPC film was required to accommodate the presence of the ctDNA, this suggests that the distribution of charges in the lipid film matches well the charge spacing of ctDNA. Brewster angle microscopy measurements of DSPC on water in the absence of Ca2+ showed the presence of a continuous film containing small, regular shaped domains at all four surface pressures examined. When Ca2+ ions were present in the sub-phase, although the film was still continuous, the domains comprising the film were more irregular in appearance while the presence of Ca2+ ions and ctDNA resulted in the domains becoming smaller and more regularly packed on the surface. © 2013, Royal Society of Chemistry.en_AU
dc.identifier.citationDabkowska, A. P., Talbot, J. P., Cavalcanti, L., Webster, J. R. P., Nelson, A., Barlow, D. J., Fragneto, G., & Lawrence, M. J. (2013). Calcium mediated interaction of calf-thymus DNA with monolayers of distearoylphosphatidylcholine: a neutron and X-ray reflectivity study. Soft Matter, 9(29), 7095-7105. doi:10.1039/c3sm50350jen_AU
dc.identifier.govdoc5079en_AU
dc.identifier.issn1744-683Xen_AU
dc.identifier.issue29en_AU
dc.identifier.journaltitleSoft Matteren_AU
dc.identifier.pagination7095-7105en_AU
dc.identifier.urihttp://dx.doi.org/10.1039/c3sm50350jen_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/5730en_AU
dc.identifier.volume9en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectNeutronsen_AU
dc.subjectX-ray lasersen_AU
dc.subjectReflectivityen_AU
dc.subjectScatteringen_AU
dc.subjectLipidsen_AU
dc.subjectAir-water interactionsen_AU
dc.titleCalcium mediated interaction of calf-thymus DNA with monolayers of distearoylphosphatidylcholine: a neutron and X-ray reflectivity studyen_AU
dc.typeJournal Articleen_AU
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