Green fluorescent protein alters the transcriptional regulation of human mitochondrial genes after gamma irradiation

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dc.contributor.authorKam, WWYen_AU
dc.contributor.authorMiddleton, RJen_AU
dc.contributor.authorLake, Ven_AU
dc.contributor.authorBanati, RBen_AU
dc.date.accessioned2013-10-24T23:30:42Zen_AU
dc.date.available2013-10-24T23:30:42Zen_AU
dc.date.issued2013-07-01en_AU
dc.date.statistics2013-10-25en_AU
dc.description.abstractGreen fluorescent proteins (GFP), extensively used as reporters in biological and imaging studies, are assumed to be mostly biologically inert. Here, we test the assumption in regard to the transcriptional regulation of 18 mitochondrially encoded genes in GFP expressing human T-cell line (JURKAT cells) exposed to gamma radiation. Using quantitative polymerase chain reaction, we demonstrate that wild type and GFP expressing JURKAT cells have different baseline mitochondrial transcript expression (10 out of the 18 tested genes) and after a single dose of radiation (100 Gy) show a significantly different transcriptional regulation of their mitochondrial genes. While in wild type cells, ten of the tested genes are up-regulated in response to radiation exposure, GFP expressing cells show less transcriptional regulation with a small down-regulation in five genes. Our results indicate that the presence of GFP in the cytoplasm can alter the cellular response to ionizing radiation.© 2013, Springer.en_AU
dc.identifier.citationKam, W. W. Y., Middleton, R., Lake, V., & Banati, R. B., (2013). Green fluorescent protein alters the transcriptional regulation of human mitochondrial genes after gamma irradiation. Journal of Fluorescence, 23 (4), 613-619. doi:10.1007/s10895-013-1206-xen_AU
dc.identifier.govdoc5056en_AU
dc.identifier.issn1053-0509en_AU
dc.identifier.issue4en_AU
dc.identifier.journaltitleJournal of Fluorescenceen_AU
dc.identifier.pagination613-619en_AU
dc.identifier.urihttp://dx.doi.org/10.1007/s10895-013-1206-xen_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/4836en_AU
dc.identifier.volume23en_AU
dc.language.isoenen_AU
dc.publisherSpringer/Plenum Publishersen_AU
dc.subjectDNAen_AU
dc.subjectActinen_AU
dc.subjectMyosinen_AU
dc.subjectIonizationen_AU
dc.subjectRadiationsen_AU
dc.subjectMutationsen_AU
dc.subjectMusclesen_AU
dc.subjectLiveren_AU
dc.titleGreen fluorescent protein alters the transcriptional regulation of human mitochondrial genes after gamma irradiationen_AU
dc.typeJournal Articleen_AU
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