Targeted detection of phosphatidylserine in biomimetic membranes and in vitro cell systems using annexin V-containing cubosomes

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dc.contributor.authorShen, HHen_AU
dc.contributor.authorLake, Ven_AU
dc.contributor.authorLe Brun, APen_AU
dc.contributor.authorJames, Men_AU
dc.contributor.authorDuff, APen_AU
dc.contributor.authorPeng, Yen_AU
dc.contributor.authorMcLean, KMen_AU
dc.contributor.authorHartley, PGen_AU
dc.date.accessioned2013-09-11T01:24:36Zen_AU
dc.date.available2013-09-11T01:24:36Zen_AU
dc.date.issued2013-11-01en_AU
dc.date.statistics2013-09-11en_AU
dc.description.abstractIn this work we have formulated Annexin V (ANX) decorated phosphatidylserine containing phytantriol (PSPhy) cubosomes to act as probes for the enhanced detection of apoptotic membranes in both model and in vitro cell systems. Small angle X-ray scattering (SAXS) and cryogenic-transmission electron microscopy (Cryo-TEM) indicated that ANX-containing PSPhy (ANX-PSPhy) cubosomes retain the Pn3m cubic symmetry and cubic phase nanoparticle characteristics of PSPhy cubosomes. The interaction of ANX-PSPhy cubosomes with apoptotic model and cellular membranes was also investigated using both quartz crystal microbalance with dissipation and confocal microscopy which confirmed that ANX-PSPhy cubosomes can selectively bind to apoptotic cells and model membranes. Neutron reflectometry has also been used to show strong binding of ANX-PSPhy cubosomes to a model apoptotic membrane, and in addition reveals changes in both the bilayer structure and in the internal structure of the cubosome in a region adjacent to the membrane as a result of material exchange. This material exchange between cubosome and apoptotic model bilayer was further demonstrated using Cryo-TEM. We have demonstrated that lipid bound protein, in this case Annexin V, can be used to target cubosome systems to biological surfaces in vitro. © 2013, Elsevier Ltd.en_AU
dc.identifier.citationShen, H.-H., Lake, V., Le Brun, A. P., James, M., Duff, A. P., Peng, Y., McLean, K. M., & Hartley, P. G. (2013). Targeted detection of phosphatidylserine in biomimetic membranes and in vitro cell systems using annexin V-containing cubosomes. Biomaterials, 34, 8361-8369. doi:10.1016/j.biomaterials.2013.07.042en_AU
dc.identifier.govdoc5045en_AU
dc.identifier.issn0142-9612en_AU
dc.identifier.journaltitleBiomaterialsen_AU
dc.identifier.pagination8361-8369en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.biomaterials.2013.07.042en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/4679en_AU
dc.identifier.volume34en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectApoptosisen_AU
dc.subjectLipidsen_AU
dc.subjectMembranesen_AU
dc.subjectLiquid crystalsen_AU
dc.subjectProbesen_AU
dc.subjectQuartzen_AU
dc.titleTargeted detection of phosphatidylserine in biomimetic membranes and in vitro cell systems using annexin V-containing cubosomesen_AU
dc.typeJournal Articleen_AU
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