Regulation of the membrane insertion and conductance activity of the metamorphic chloride intracellular channel protein CLIC1 by cholesterol

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dc.contributor.authorValenzuela, SMen_AU
dc.contributor.authorAlkhamici, Hen_AU
dc.contributor.authorBrown, LJen_AU
dc.contributor.authorAlmond, OCen_AU
dc.contributor.authorGoodchild, SCen_AU
dc.contributor.authorCarne, Sen_AU
dc.contributor.authorCurmi, PMGen_AU
dc.contributor.authorHolt, SAen_AU
dc.contributor.authorCornell, BAen_AU
dc.date.accessioned2014-06-26T01:49:00Zen_AU
dc.date.available2014-06-26T01:49:00Zen_AU
dc.date.issued2013-02-14en_AU
dc.date.statistics2014-06-26en_AU
dc.description.abstractThe Chloride Intracellular ion channel protein CLIC1 has the ability to spontaneously insert into lipid membranes from a soluble, globular state. The precise mechanism of how this occurs and what regulates this insertion is still largely unknown, although factors such as pH and redox environment are known contributors. In the current study, we demonstrate that the presence and concentration of cholesterol in the membrane regulates the spontaneous insertion of CLIC1 into the membrane as well as its ion channel activity. The study employed pressure versus area change measurements of Langmuir lipid monolayer films; and impedance spectroscopy measurements using tethered bilayer membranes to monitor membrane conductance during and following the addition of CLIC1 protein. The observed cholesterol dependent behaviour of CLIC1 is highly reminiscent of the cholesterol-dependent-cytolysin family of bacterial pore-forming proteins, suggesting common regulatory mechanisms for spontaneous protein insertion into the membrane bilayer. © 2013, Valenzuela et al.en_AU
dc.description.sponsorshipAcquired from www.plos.org (Public Library of Science)under Creative Commons Attribution (CC BY) license.en_AU
dc.identifier.articlenumbere56948en_AU
dc.identifier.citationValenzuela, S. M., Alkhamici, H., Brown, L. J., Almond, O. C., Goodchild, S. C., Carne, S., Curmi, P. M. G., Holt, S. A., & Cornell, B. A. (2013). Regulation of the membrane insertion and conductance activity of the metamorphic chloride intracellular channel protein CLIC1 by cholesterol. PLoS ONE, 8(2), e56948. doi:10.1371/journal.pone.0056948en_AU
dc.identifier.govdoc4893en_AU
dc.identifier.isbn1932-6203en_AU
dc.identifier.issn1932-6203en_AU
dc.identifier.issue2en_AU
dc.identifier.journaltitlePLoS ONEen_AU
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0056948en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/5720en_AU
dc.identifier.volume8en_AU
dc.language.isoenen_AU
dc.publisherPublic Library of Scienceen_AU
dc.subjectCrystalsen_AU
dc.subjectCrystal structureen_AU
dc.subjectLipidsen_AU
dc.subjectFluorescenceen_AU
dc.subjectIonsen_AU
dc.subjectSolubilityen_AU
dc.titleRegulation of the membrane insertion and conductance activity of the metamorphic chloride intracellular channel protein CLIC1 by cholesterolen_AU
dc.typeJournal Articleen_AU
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