Mimicking the receptor-aided binding of HIV-1 TAT protein transduction domains to phospholipid monolayers at the air-water interface

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dc.contributor.authorHong, Den_AU
dc.contributor.authorShin, Ken_AU
dc.contributor.authorJames, Men_AU
dc.contributor.authorTae, Gen_AU
dc.date.accessioned2014-04-17T04:57:34Zen_AU
dc.date.available2014-04-17T04:57:34Zen_AU
dc.date.issued2012-01-01en_AU
dc.date.statistics2014-04-17en_AU
dc.description.abstractWe have designed heparin-incorporated model lipid monolayers and monitored the adsorption behaviours of cell penetrating peptides (CPPs) on a molecular scale at the air-water interface. We found initially that heparin could incorporate homogeneously into 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphoserine (DPPS), and DPPC/DPPS mixed monolayers, allowing improved adsorption of transcription-activating factor (TAT) derived peptide (TAT-TDP) molecules. X-ray reflectivity measurements, as well as the surface pressure changes from surface pressure-area isotherms, suggest that a preferred interaction of heparin with TAT-TDP occurs, and is responsible for the effective penetration. This behaviour resembles the ubiquitous activities of glycosaminoglycan (GAG) molecules as cellular receptors that promote intracellular transport of cell-penetrating peptide domains in biological systems. We suggest that heparin-TAT-TDP complex formation can be exploited in a primary step of CPP translocation. © 2012, Royal Society of Chemistry.en_AU
dc.identifier.citationHong, D., Shin, K., James, M., & Tae, G. (2012). Mimicking the receptor-aided binding of HIV-1 TAT protein transduction domains to phospholipid monolayers at the air-water interface. Soft Matter, 8(33), 8616-8623. doi:10.1039/c2sm25885den_AU
dc.identifier.govdoc4630en_AU
dc.identifier.issn1744-683Xen_AU
dc.identifier.issue33en_AU
dc.identifier.journaltitleSoft Matteren_AU
dc.identifier.pagination8616-8623en_AU
dc.identifier.urihttp://dx.doi.org/10.1039/c2sm25885den_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/5450en_AU
dc.identifier.volume8en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectPeptidesen_AU
dc.subjectX-ray lasersen_AU
dc.subjectLipidsen_AU
dc.subjectMembranesen_AU
dc.subjectSurfacesen_AU
dc.subjectIsothermsen_AU
dc.titleMimicking the receptor-aided binding of HIV-1 TAT protein transduction domains to phospholipid monolayers at the air-water interfaceen_AU
dc.typeJournal Articleen_AU
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