In situ neutron reflectivity studies of the adsorption of DNA by charged diblock copolymer monolayers at the air-water interface

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dc.contributor.authorYang, PWen_AU
dc.contributor.authorLin, TLen_AU
dc.contributor.authorLiu, ITen_AU
dc.contributor.authorHu, Yen_AU
dc.contributor.authorJames, Men_AU
dc.date.accessioned2014-04-22T06:18:43Zen_AU
dc.date.available2014-04-22T06:18:43Zen_AU
dc.date.issued2012-01-01en_AU
dc.date.statistics2014-04-22en_AU
dc.description.abstractThe absorption of 700 bp DNA by a charged diblock copolymer monolayer at the air-water (D2O) interface was investigated by surface pressure measurements and neutron reflectometry. The amphiphilic diblock copolymer PS-P4VPQ with molecular weights of 19 600 and 12 000, respectively, formed a stable monolayer at the air-water interface, with PS blocks in a highly packed layer at the surface, a dense P4VPQ brush layer adjacent to the PS and a loose P4VPQ brush layer extending into the aqueous subphase. Most of the P4VPQ segments were distributed in the dense brush layer and only 7% of the P4VPQ segments were contained in the loose brush layer. Neutron reflectometry revealed that the distinct two-layer brush structure remained essentially unchanged even upon adsorption of large amounts of DNA (up to a volume fraction of similar to 29%). As compared with the DNA adsorption by cationic lipid monolayers, the PS-P4VPQ monolayer has a higher capability to adsorb DNA through its three-dimensionally structured brushes. In the presence of calcium ions in the subphase, the DNA adsorption in both the dense and loose brush layers was significantly enhanced (up to a volume fraction of similar to 73%). For a calcium ion concentration of 80 mM, an additional DNA condensation layer was observed below the loose P4VPQ brush layer. © 2012, Royal Society of Chemistry.en_AU
dc.identifier.citationYang, P. W., Lin, T. L., Liu, I. T., Hu, Y., & James, M. (2012). In situ neutron reflectivity studies of the adsorption of DNA by charged diblock copolymer monolayers at the air-water interface. Soft Matter, 8(27), 7161-7168. doi:10.1039/c2sm25297jen_AU
dc.identifier.govdoc4446en_AU
dc.identifier.issn1744-683Xen_AU
dc.identifier.issue27en_AU
dc.identifier.journaltitleSoft Matteren_AU
dc.identifier.pagination7161en_AU
dc.identifier.urihttp://dx.doi.org/10.1039/c2sm25297jen_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/5465en_AU
dc.identifier.volume8en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectAir-water interactionsen_AU
dc.subjectCopolymersen_AU
dc.subjectNeutronsen_AU
dc.subjectIonsen_AU
dc.subjectCalcium ionsen_AU
dc.subjectDNAen_AU
dc.titleIn situ neutron reflectivity studies of the adsorption of DNA by charged diblock copolymer monolayers at the air-water interfaceen_AU
dc.typeJournal Articleen_AU
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