Bilayer-mediated clustering and functional interaction of MscL channels

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dc.contributor.authorGrage, SLen_AU
dc.contributor.authorKeleshian, AMen_AU
dc.contributor.authorTurdzeladze, Ten_AU
dc.contributor.authorBattle, ARen_AU
dc.contributor.authorTay, WCen_AU
dc.contributor.authorMay, RPen_AU
dc.contributor.authorHolt, SAen_AU
dc.contributor.authorContera, SAen_AU
dc.contributor.authorHaertlein, Men_AU
dc.contributor.authorMoulin, Men_AU
dc.contributor.authorPal, Pen_AU
dc.contributor.authorRohde, PRen_AU
dc.contributor.authorForsyth, VTen_AU
dc.contributor.authorWatts, Aen_AU
dc.contributor.authorHuang, KCen_AU
dc.contributor.authorUlrich, ASen_AU
dc.contributor.authorMartinac, Ben_AU
dc.date.accessioned2011-12-05T23:53:21Zen_AU
dc.date.available2011-12-05T23:53:21Zen_AU
dc.date.issued2011-03-02en_AU
dc.date.statistics2011-12-06en_AU
dc.description.abstractMechanosensitive channels allow bacteria to respond to osmotic stress by opening a nanometer-sized pore in the cellular membrane. Although the underlying mechanism has been thoroughly studied on the basis of individual channels, the behavior of channel ensembles has yet to be elucidated. This work reveals that mechanosensitive channels of large conductance (MscL) exhibit a tendency to spatially cluster, and demonstrates the functional relevance of clustering. We evaluated the spatial distribution of channels in a lipid bilayer using patch-clamp electrophysiology, fluorescence and atomic force microscopy, and neutron scattering and reflection techniques, coupled with mathematical modeling of the mechanics of a membrane crowded with proteins. The results indicate that MscL forms clusters under a wide range of conditions. MscL is closely packed within each cluster but is still active and mechanosensitive. However, the channel activity is modulated by the presence of neighboring proteins, indicating membrane-mediated protein-protein interactions. Collectively, these results suggest that MscL self-assembly into channel clusters plays an osmoregulatory functional role in the membrane.© 2011, Cell Pressen_AU
dc.identifier.citationGrage, S. L., Keleshian, A. M., Turdzeladze, T., Battle, A. R., Tay, W. C., May, R. P., Holt, S. A., Contera, S. A., Haertlein, M., Moulin, M., Pal, P., Rohde, P. R., Forsyth, V. T., Watts, A., Huang, K. C., Ulrich, A. S., & Martinac, B. (2011). Bilayer-mediated clustering and functional interaction of MscL channels. Biophysical Journal, 100(5), 1252-1260. doi:10.1016/j.bpj.2011.01.023en_AU
dc.identifier.govdoc3883en_AU
dc.identifier.issn0006-3495en_AU
dc.identifier.issue5en_AU
dc.identifier.journaltitleBiophysical Journalen_AU
dc.identifier.pagination1252-1260en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.bpj.2011.01.023en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/3914en_AU
dc.identifier.volume100en_AU
dc.language.isoenen_AU
dc.publisherCell Pressen_AU
dc.subjectMembranesen_AU
dc.subjectProteinsen_AU
dc.subjectReceptorsen_AU
dc.subjectNeutron diffractionen_AU
dc.subjectIon pairsen_AU
dc.subjectBacteriaen_AU
dc.titleBilayer-mediated clustering and functional interaction of MscL channelsen_AU
dc.typeJournal Articleen_AU
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