Amylolysis of wheat starches. II. Degradation patterns of native starch granules with varying functional properties

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dc.contributor.authorBlazek, Jen_AU
dc.contributor.authorCopeland, Len_AU
dc.date.accessioned2010-11-03T02:54:56Zen_AU
dc.date.available2010-11-03T02:54:56Zen_AU
dc.date.issued2010-09-01en_AU
dc.date.statistics2010-09-01en_AU
dc.description.abstractScanning electron microscopy (SEM) and X-ray diffraction (XRD) were employed to investigate degradation patterns of native starch granules from wheat (Triticum aestivum L.) by different starch-degrading enzymes. The starches examined were from a waxy wheat and four varieties with slightly elevated amylose content, but with different functional properties. Differences in the digestion patterns after partial α-amylolysis of starch granules were noted between the starches. The waxy starch seemed to be degraded by endocorrosion, whereas the amylose-rich starches followed a slower mode of hydrolysis starting from the granular surface. X-ray diffractograms of the amylose-rich starches were not significantly altered by 2 h of α-amylolysis, whereas partial hydrolysis of the waxy starch decreased scattering intensity at higher 2θ angles, consistent with a different mode of attack by α-amylase in the initial digestion stages of granules of waxy and amylose-rich starches. We propose these differences are due to the combined effects of the change in packing density and partial preference for hydrolysis of amorphous material. The native starch granules were also attacked by beta-amylase, isoamylase and amyloglucosidase, which indicates that α-amylase is not the only starch-degrading enzyme that is able to initiate starch hydrolysis of native granules. © 2010, Elsevier Ltd.en_AU
dc.identifier.citationBlazek, J., & Copeland, L. (2010). Amylolysis of wheat starches. II. Degradation patterns of native starch granules with varying functional properties. Journal of Cereal Science, 52(2), 295-302. doi:10.1016/j.jcs.2010.06.011en_AU
dc.identifier.govdoc2982en_AU
dc.identifier.issn0733-5210en_AU
dc.identifier.issue2en_AU
dc.identifier.journaltitleJournal of Cereal Scienceen_AU
dc.identifier.pagination295-302en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.jcs.2010.06.011en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2803en_AU
dc.identifier.volume52en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectWheaten_AU
dc.subjectStarchen_AU
dc.subjectScanning electron microscopyen_AU
dc.subjectX-ray diffractionen_AU
dc.subjectHydrolysisen_AU
dc.subjectDigestionen_AU
dc.titleAmylolysis of wheat starches. II. Degradation patterns of native starch granules with varying functional propertiesen_AU
dc.typeJournal Articleen_AU
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