Small-angle scattering for structural biology - expanding the frontier while avoiding the pitfalls

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dc.contributor.authorJacques, DAen_AU
dc.contributor.authorTrewhella, Jen_AU
dc.date.accessioned2010-05-11T00:30:37Zen_AU
dc.date.available2010-05-11T00:30:37Zen_AU
dc.date.issued2010-04en_AU
dc.date.statistics2010-04en_AU
dc.description.abstractThe last decade has seen a dramatic increase in the use of small-angle scattering for the study of biological macromolecules in solution. The drive for more complete structural characterization of proteins and their interactions, coupled with the increasing availability of instrumentation and easy-to-use software for data analysis and interpretation, is expanding the utility of the technique beyond the domain of the biophysicist and into the realm of the protein scientist. However, the absence of publication standards and the ease with which 3D models can be calculated against the inherently 1D scattering data means that an understanding of sample quality, data quality, and modeling assumptions is essential to have confidence in the results. This review is intended to provide a road map through the small-angle scattering experiment, while also providing a set of guidelines for the critical evaluation of scattering data. Examples of current best practice are given that also demonstrate the power of the technique to advance our understanding of protein structure and function. © 2010, Wiley-Blackwell.en_AU
dc.identifier.citationJacques, D. A., & Trewhella, J. (2010). Small-angle scattering for structural biology - expanding the frontier while avoiding the pitfalls. Protein Science, 19(4), 642-657. doi:10.1002/pro.351en_AU
dc.identifier.govdoc1681en_AU
dc.identifier.issn0961-8368en_AU
dc.identifier.issue4en_AU
dc.identifier.journaltitleProtein Scienceen_AU
dc.identifier.pagination642-657en_AU
dc.identifier.urihttp://dx.doi.org/10.1002/pro.351en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/1661en_AU
dc.identifier.volume19en_AU
dc.language.isoenen_AU
dc.publisherWiley-Blackwellen_AU
dc.subjectSmall angle scatteringen_AU
dc.subjectProtein structureen_AU
dc.subjectBiologyen_AU
dc.subjectData analysisen_AU
dc.subjectInteractionsen_AU
dc.subjectSimulationen_AU
dc.titleSmall-angle scattering for structural biology - expanding the frontier while avoiding the pitfallsen_AU
dc.typeJournal Articleen_AU
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