Gamma irradiation as a biological decontaminant and its effect on common fingermark detection techniques and DNA profiling.

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dc.contributor.authorHoile, Ren_AU
dc.contributor.authorBanos, Cen_AU
dc.contributor.authorColella, Men_AU
dc.contributor.authorWalsh, SJen_AU
dc.contributor.authorRoux, Cen_AU
dc.date.accessioned2010-04-19T06:52:15Zen_AU
dc.date.accessioned2010-04-30T05:08:45Zen_AU
dc.date.available2010-04-19T06:52:15Zen_AU
dc.date.available2010-04-30T05:08:45Zen_AU
dc.date.issued2010-01en_AU
dc.date.statistics2010-01en_AU
dc.description.abstractThe use of disease-causing organisms and their toxins against the civilian population has defined bioterrorism and opened forensic science up to the challenges of processing contaminated evidence. This study sought to determine the use of gamma irradiation as an effective biological decontaminant and its effect on the recovery of latent fingermarks from both porous and nonporous items. Test items were contaminated with viable spores marked with latent prints and then decontaminated using a cobalt 60 gamma irradiator. Fingermark detection was the focus with standard methods including 1,2-indanedione, ninhydrin, diazafluoren-9-one, and physical developer used during this study. DNA recovery using 20% Chelex extraction and quantitative real-time polymerase chain reaction was also explored. Gamma irradiation proved effective as a bacterial decontaminant with D-values ranging from 458 to 500 Gy for nonporous items and 797–808 Gy for porous ones. The results demonstrated the successful recovery of latent marks and DNA establishing gamma irradiation as a viable decontamination option. © 2010, Wiley-Blackwell.en_AU
dc.identifier.citationHoile, R., Banos, C., Colella, M., Walsh, S. J., & Roux, C. (2010). Gamma irradiation as a biological decontaminant and its effect on common fingermark detection techniques and DNA profiling. Journal of Forensic Sciences, 55(1), 171-177. doi:10.1111/j.1556-4029.2009.01233.xen_AU
dc.identifier.govdoc1563en_AU
dc.identifier.issn0022-1198en_AU
dc.identifier.issue1en_AU
dc.identifier.journaltitleJournal of Forensic Sciencesen_AU
dc.identifier.pagination171-177en_AU
dc.identifier.urihttp://dx.doi.org/10.1111/j.1556-4029.2009.01233.xen_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/3214en_AU
dc.identifier.volume55en_AU
dc.language.isoenen_AU
dc.publisherWiley-Blackwellen_AU
dc.subjectCrime detectionen_AU
dc.subjectDNAen_AU
dc.subjectIrradiationen_AU
dc.subjectDecontaminationen_AU
dc.subjectFingersen_AU
dc.subjectLatent imagesen_AU
dc.titleGamma irradiation as a biological decontaminant and its effect on common fingermark detection techniques and DNA profiling.en_AU
dc.typeJournal Articleen_AU
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