Different views from small angles.

Trewhella, J

Different views from small angles.

Date

2008-04-01

Authors

Trewhella, J

Publisher

National Academy of Sciences

Abstract

The small-angle scattering of x-rays or neutrons from proteins in solution can provide important information about the structure of the protein and the nature of interactions or distance correlations among the protein molecules (1, 2). The former is encoded in the form factor [P(q)], and the latter in the structure factor [S(q)]. These functions are of great interest to the structural biology community; the form factor can be used to develop and test three-dimensional structural models of proteins (3, 4), whereas the structure factor can inform efforts to crystallize proteins for high-resolution structural analysis by providing insights into their organization in solution (4, 5). Because the measured small-angle scattering profile [I(q)] from a solution of particles is proportional to the product of the ensemble and rotationally averaged form factor and the structure factor [P(q)S(q)], accurate extraction of the two contributing functions, and their subsequent interpretation, is complex. The work of Shukla et al. reported in this issue of PNAS (6) is aimed at resolving controversy with regard to the interpretation of small-angle scattering data from solutions of the well studied protein lysozyme. The specific issue examined concerns the interpretation of the extracted S(q) in terms of intermolecular interactions among lysozyme molecules. The conclusion drawn by Shukla et al. contradicts previously published interpretations of similar data and is boldly presented as their title: "Absence of equilibrium cluster phase in concentrated lysozyme solutions." © 2008, National Academy of Sciences

Keywords

Small angle scattering, Neutrons, Proteins, Molecules, Lysozyme, Interactions

Citation

Trewhella, J. (2008). The different views from small angles. Proceedings of the National Academy of Sciences, 105(13), 4967-4968. doi:10.1073/pnas.0801324105