Cortactin adopts a globular conformation and bundles actin into sheets

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dc.contributor.authorCowieson, NPen_AU
dc.contributor.authorKing, GJen_AU
dc.contributor.authorCookson, DJen_AU
dc.contributor.authorRoss, Ien_AU
dc.contributor.authorHuber, Ten_AU
dc.contributor.authorHume, DAen_AU
dc.contributor.authorKobe, Ben_AU
dc.contributor.authorMartin, JLen_AU
dc.date.accessioned2009-01-28T01:30:31Zen_AU
dc.date.accessioned2010-04-30T05:06:51Zen_AU
dc.date.available2009-01-28T01:30:31Zen_AU
dc.date.available2010-04-30T05:06:51Zen_AU
dc.date.issued2008-06-06en_AU
dc.date.statistics2008-06-06en_AU
dc.description.abstractCortactin is a filamentous actin-binding protein that plays a pivotal role in translating environmental signals into coordinated rearrangement of the cytoskeleton. The dynamic reorganization of actin in the cytoskeleton drives processes including changes in cell morphology, cell migration, and phagocytosis. In general, structural proteins of the cytoskeleton bind in the N-terminal region of cortactin and regulatory proteins in the C-terminal region. Previous structural studies have reported an extended conformation for cortactin. It is therefore unclear how cortactin facilitates cross-talk between structural proteins and their regulators. In the study presented here, circular dichroism, chemical cross-linking, and small angle x-ray scattering are used to demonstrate that cortactin adopts a globular conformation, thereby bringing distant parts of the molecule into close proximity. In addition, the actin bundling activity of cortactin is characterized, showing that fully polymerized actin filaments are bundled into sheet-like structures. We present a low resolution structure that suggests how the various domains of cortactin interact to coordinate its array of binding partners at sites of actin branching. © 2008, American Society for Biochemistry and Molecular Biologyen_AU
dc.identifier.citationCowieson, N. P., King, G., Cookson, D., Ross, I., Huber, T., Hume, D. A., Kobe, B., & Martin, J. L. (2008). Cortactin adopts a globular conformation and bundles actin into sheets. Journal of Biological Chemistry, 283(23), 16187-16193. doi:doi:10.1074/jbc.M708917200en_AU
dc.identifier.govdoc1438en_AU
dc.identifier.issn0021-9258en_AU
dc.identifier.issue23en_AU
dc.identifier.journaltitleJournal of Biological Chemistryen_AU
dc.identifier.pagination16187-16193en_AU
dc.identifier.urihttp://dx.doi.org/doi:10.1074/jbc.M708917200en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/1258en_AU
dc.identifier.volume228en_AU
dc.language.isoenen_AU
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen_AU
dc.subjectProteinsen_AU
dc.subjectActinen_AU
dc.subjectSkeletonen_AU
dc.subjectCell culturesen_AU
dc.subjectPhagocytosisen_AU
dc.subjectSheetsen_AU
dc.titleCortactin adopts a globular conformation and bundles actin into sheetsen_AU
dc.typeJournal Articleen_AU
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