In vivo deuteration of a native bacterial biopolymer for structural elucidation using SANS

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dc.contributor.authorHolden, PJen_AU
dc.contributor.authorRussell, RAen_AU
dc.contributor.authorStone, DJMen_AU
dc.contributor.authorGarvey, CJen_AU
dc.contributor.authorFoster, LJRen_AU
dc.date.accessioned2009-10-27T01:17:29Zen_AU
dc.date.accessioned2010-04-30T05:06:41Zen_AU
dc.date.available2009-10-27T01:17:29Zen_AU
dc.date.available2010-04-30T05:06:41Zen_AU
dc.date.issued2004-07-15en_AU
dc.date.statistics2004-07-15en_AU
dc.description.abstractIn order to facilitate future structural studies, biodeuteration of bacterial polyhydroxyalkanoates (PHAs) was investigated. We report here the in vivo deuteration of poly 3-hydroxyoctanoate (PHO) produced by its native host, the bacterium Pseudomonas oleovorans. Bacterial biomass was produced in bioreactor studies by growth on hydrogenated substrates and PHO was subsequently produced intracellularly (10–20% w/w) during batch fed growth on deuterated octanoic acid under oxygen limitation. GC-MS analyses of the PHO demonstrated that 13 of the 15 hydrogen atoms had been replaced with deuterium (except in position 3), the remaining two hydrogen presumably being derived from water. A SANS contrast variation study was conducted on whole cells and the results indicate the potential to discriminate inclusion bodies formed from deuterated precursor from an otherwise hydrogenated background. © 2004, Elsevier Ltd.en_AU
dc.identifier.citationHolden, P. J., Russell, R. A., Stone, D. J. M., Garvey, C. J., & Foster, L. J. R. (2004). In vivo deuteration of a native bacterial biopolymer for structural elucidation using SANS. Paper presented to the Third European Conference on Neutron Scattering (ENS 2003), 3-6 September 2003, Montpellier, France, In H. Mutka (Ed), Physica B: Condensed Matter, 350(1-3), E643-E646. doi:10.1016/j.physb.2004.03.172en_AU
dc.identifier.conferenceenddate6 September 2003en_AU
dc.identifier.conferencenameThird European Conference on Neutron Scattering (ENS 2003)en_AU
dc.identifier.conferenceplaceMontpellieren_AU
dc.identifier.conferencestartdate3 September 2003en_AU
dc.identifier.editorsH. Mutkaen_AU
dc.identifier.govdoc1429en_AU
dc.identifier.issn0921-4526en_AU
dc.identifier.issue1-3en_AU
dc.identifier.journaltitlePhysica B: Condensed Matteren_AU
dc.identifier.paginationE643-E646en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.physb.2004.03.172en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2090en_AU
dc.identifier.volume350en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectDeuterationen_AU
dc.subjectSmall angle scatteringen_AU
dc.subjectHydrogenationen_AU
dc.subjectDeuteriumen_AU
dc.subjectIn vivoen_AU
dc.subjectBacteriaen_AU
dc.titleIn vivo deuteration of a native bacterial biopolymer for structural elucidation using SANSen_AU
dc.typeConference Paperen_AU
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