Shear-induced alignment of self-associated hemoglobin in human erythrocytes: small angle neutron scattering studies

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dc.contributor.authorGarvey, CJen_AU
dc.contributor.authorKnott, RBen_AU
dc.contributor.authorDrabarek, Een_AU
dc.contributor.authorKuchel, PWen_AU
dc.date.accessioned2009-11-24T03:46:53Zen_AU
dc.date.accessioned2010-04-30T05:06:39Zen_AU
dc.date.available2009-11-24T03:46:53Zen_AU
dc.date.available2010-04-30T05:06:39Zen_AU
dc.date.issued2004-11en_AU
dc.date.statistics2004-11en_AU
dc.description.abstractSmall angle neutron scattering (SANS) was performed on suspensions of actively metabolising human erythrocytes in the constant shear field induced by a Couette cell. The SANS pattern recorded on a two-dimensional detector was a function of the shear rate; at zero shear, the SANS pattern had radial symmetry around the direction of the beam. The radial average of the SANS pattern consisted of a broad intensity maximum superimposed on a decay. The intensity maximum at q = 0.1 Å(-1) supercript stop was attributed to isotropically oriented self-associated complexes of the tetrameric oxygen transport protein hemoglobin inside the erythrocytes. A flow curve of the cell suspension was used to identify at what shear rate a suspension of uniaxially oriented ellipsoidal cells is produced. The radial symmetry of the SANS patterns persisted until the shear rate was sufficient to produce a suspension of uniaxially oriented ellipsoidal cells. Again, an intensity maximum was present in directions parallel and orthogonal to the shear axis, but this intensity maximum was superimposed upon quite different intensity decays in each direction from that of the primary neutron beam. The angular range of the SANS instrument was limited, however the results from shear-induced structural changes is consistent with a model that involves hemoglobin complexes that are aligned with respect to the plasma membranes of the elongated cells. © 2004, Springer.en_AU
dc.identifier.citationGarvey, C. J., Knott, R. B., Drabarek, E., & Kuchel, P. W. (2004). Shear-induced alignment of self-associated hemoglobin in human erythrocytes: small angle neutron scattering studies. European Biophysics Journal with Biophysics Letters, 33(7), 589-595. doi:10.1007/s00249-004-0408-1en_AU
dc.identifier.govdoc1427en_AU
dc.identifier.issn0175-7571en_AU
dc.identifier.issue7en_AU
dc.identifier.journaltitleEuropean Biophysics Journal with Biophysics Lettersen_AU
dc.identifier.pagination589-595en_AU
dc.identifier.urihttp://dx.doi.org/10.1007/s00249-004-0408-1en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2475en_AU
dc.identifier.volume33en_AU
dc.language.isoenen_AU
dc.publisherSpringeren_AU
dc.subjectSmall angle scatteringen_AU
dc.subjectShearen_AU
dc.subjectErythrocytesen_AU
dc.subjectHemoglobinen_AU
dc.subjectHydrodynamicsen_AU
dc.subjectAlignmenten_AU
dc.titleShear-induced alignment of self-associated hemoglobin in human erythrocytes: small angle neutron scattering studiesen_AU
dc.typeJournal Articleen_AU
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