Investigation of the phase morphology of bacterial PHA inclusion bodies by contrast variation SANS

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dc.contributor.authorRussell, RAen_AU
dc.contributor.authorHolden, PJen_AU
dc.contributor.authorGarvey, CJen_AU
dc.contributor.authorWilde, KLen_AU
dc.contributor.authorHammerton, KMen_AU
dc.contributor.authorFoster, LJRen_AU
dc.date.accessioned2008-04-11T07:00:34Zen_AU
dc.date.accessioned2010-04-30T05:06:38Zen_AU
dc.date.available2008-04-11T07:00:34Zen_AU
dc.date.available2010-04-30T05:06:38Zen_AU
dc.date.issued2006-11-15en_AU
dc.date.statistics2006-11en_AU
dc.description.abstractUnder growth-limiting conditions, many bacteria are able to metabolise excess organic acids into polyhydroxyalkanoates (PHA) and store these polymers as intracellular inclusions until the return of favourable conditions. Various models have been proposed for the macromolecular organisation of the boundary layer Surrounding the polymer, and contrast-variation small-angle neutron scattering (SANS) was used to study its organisation. Inclusions formed by Pseudomonas oleovorans under hydrogenating conditions showed lowest scattering intensity at ca. 20% D2O. The inclusions consist of protein and membrane lipids in the boundary layer and polyhydroxyoctanoate (lipid) in the inclusion body. At 20% D2O the contributions of lipids were contrast matched with the solvent, indicating that lipids contributed the bulk of the scattering intensity observed at other D2O/H2O ratios. These results are inconsistent with a model of the boundary layer which proposed outer and inner layers of crystalline protein lattice sandwiching a membrane lipid membrane layer [E.S. Stuart, R.W. Lenz, R.C. Fuller, Can J Microbiol 41(Suppl 1) (1995) 84 93], and is more consistent with a model consisting of a lipid monolayer containing embedded proteins [U. Pieper-furst, M.H. Madkour, F. Mayer, A. Steinbuchel, J. Bacteriol. 176 (1994) 4328-4337.] By altering the H/D content of the precursors, we were able to collect SANS data from preparations of both deuterated and H/D copolymer inclusions, where initial PHA produced was hydrogenated followed by deuteration. Deuterated inclusions showed minimum intensity above 90% D2O/H2O whereas the sequentially produced copolymer (assumed to be in a core/shell arrangement) displayed minimum scattering some 20% lower, which is consistent with the increased hydrogenation of the boundary layer expected from its synthesis during supply of hydrogenated followed by deuterated precursors. © 2006, Elsevier Ltden_AU
dc.identifier.citationRussell, R. A., Holden, P. J., Garvey, C. J., Wilde, K. L., Hammerton, K. M., & Foster, L. J. (2006). Investigation of the phase morphology of bacterial PHA inclusion bodies by contrast variation SANS. Paper presented at the Eighth International Conference on Neutron Scattering (ICNS 2005), "Neutrons for structure and dynamics - a new era", Sydney, Australia, 27 November to 2 December 2005. In Campbell, S. J., Cadogan, J. M., Furusaka, M., Hauser, N., & James, M. (Eds), Physica B: Condensed Matter, 385-386(Part 2), 859-861. doi:10.1016/j.physb.2006.05.126en_AU
dc.identifier.conferenceenddate2 December 2005en_AU
dc.identifier.conferencenameEighth International Conference on Neutron Scattering (ICNS 2005), "Neutrons for structure and dynamics - a new era"en_AU
dc.identifier.conferenceplaceSydney, Australiaen_AU
dc.identifier.conferencestartdate27 November 2005en_AU
dc.identifier.editorsCampbell, S. J., Cadogan, J. M., Furusaka, M., Hauser, N., & James, M.en_AU
dc.identifier.govdoc1425en_AU
dc.identifier.issn0921-4526en_AU
dc.identifier.issuePart 2en_AU
dc.identifier.journaltitlePhysica B: Condensed Matteren_AU
dc.identifier.pagination859-861en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.physb.2006.05.126en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/1063en_AU
dc.identifier.volume385-386en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectMorphologyen_AU
dc.subjectBacteriaen_AU
dc.subjectOrganic acidsen_AU
dc.subjectPolymersen_AU
dc.subjectBoundary layersen_AU
dc.subjectSmall angle scatteringen_AU
dc.titleInvestigation of the phase morphology of bacterial PHA inclusion bodies by contrast variation SANSen_AU
dc.typeConference Paperen_AU
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