Evaluation of specimen preparation techniques for micro-PIXE localisation of elements in hyperaccumulating plants
dc.contributor.author | Kachenko, AG | en_AU |
dc.contributor.author | Siegele, R | en_AU |
dc.contributor.author | Bhatia, NP | en_AU |
dc.contributor.author | Singh, B | en_AU |
dc.contributor.author | Ionescu, M | en_AU |
dc.date.accessioned | 2009-11-16T05:45:19Z | en_AU |
dc.date.accessioned | 2010-04-30T05:06:08Z | en_AU |
dc.date.available | 2009-11-16T05:45:19Z | en_AU |
dc.date.available | 2010-04-30T05:06:08Z | en_AU |
dc.date.issued | 2008-04 | en_AU |
dc.date.statistics | 2008-04 | en_AU |
dc.description.abstract | Hybanthus floribundus subsp. floribundus, a rare Australian Ni-hyperaccumulating shrub and Pityrogramma calomelanos var. austroamericana, an Australian naturalized As-hyperaccumulating fern are promising species for use in phytoremediation of contaminated sites. Micro-proton-induced X-ray emission (μ-PIXE) spectroscopy was used to map the elemental distribution of the accumulated metal(loid)s, Ca and K in leaf or pinnule tissues of the two plant species. Samples were prepared by two contrasting specimen preparation techniques: freeze-substitution in tetrahydrofuran (THF) and freeze-drying. The specimens were analysed to compare the suitability of each technique in preserving (i) the spatial elemental distribution and (ii) the tissue structure of the specimens. Further, the μ-PIXE results were compared with concentration of elements in the bulk tissue obtained by ICP-AES analysis. In H. floribundus subsp. floribundus, μ-PIXE analysis revealed Ni, Ca and K concentrations in freeze-dried leaf tissues were at par with bulk tissue concentrations. Elemental distribution maps illustrated that Ni was preferentially localised in the adaxial epidermal tissues (1% DW) and least concentration was found in spongy mesophyll tissues (0.53% DW). Conversely, elemental distribution maps of THF freeze-substituted tissues indicated significantly lower Ni, Ca and K concentrations than freeze-dried specimens and bulk tissue concentrations. Moreover, Ni concentrations were uniform across the whole specimen and no localisation was observed. In P. calomelanos var. austroamericana freeze-dried pinnule tissues, μ-PIXE revealed statistically similar As, Ca and K concentrations as compared to bulk tissue concentrations. Elemental distribution maps showed that As localisation was relatively uniform across the whole specimen. Once again, THF freeze-substituted tissues revealed a significant loss of As compared to freeze-dried specimens and the concentrations obtained by bulk tissue analysis. The results demonstrate that freeze-drying is a suitable sample preparation technique to study elemental distribution of ions in H. floribundus and P. calomelanos plant tissues using μ-PIXE spectroscopy. Furthermore, cellular structure was preserved in samples prepared using this technique. © 2007, Elsevier Ltd. | en_AU |
dc.identifier.citation | Kachenko, A. G., Siegele, R., Bhatia, N. P., Singh, B., & Ionescu, M. (2008). Evaluation of specimen preparation techniques for micro-PIXE localisation of elements in hyperaccumulating plants. Paper presented to Eighteenth International Conference on Ion Beam Analysis Eighteenth International Conference on Ion Beam Analysis (IBA 2007), 23-28 September 2007, Hyderabad, India. In A.P. Pathak, D. K. Avasthi, & B. N. Dev (Eds), Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 266(8), 1598-1604. doi:10.1016/j.nimb.2007.11.017 | en_AU |
dc.identifier.conferenceenddate | 28 September 2007 | en_AU |
dc.identifier.conferencename | Eighteenth International Conference on Ion Beam Analysis Eighteenth International Conference on Ion Beam Analysis (IBA 2007) | en_AU |
dc.identifier.conferenceplace | Hyderabad, India | en_AU |
dc.identifier.conferencestartdate | 23 September 2007 | en_AU |
dc.identifier.editors | A.P. Pathak, D. K. Avasthi, & B. N. Dev | en_AU |
dc.identifier.govdoc | 1393 | en_AU |
dc.identifier.issn | 0168-583X | en_AU |
dc.identifier.issue | 8 | en_AU |
dc.identifier.journaltitle | Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms | en_AU |
dc.identifier.pagination | 1598-1604 | en_AU |
dc.identifier.uri | http://dx.doi.org/10.1016/j.nimb.2007.11.017 | en_AU |
dc.identifier.uri | http://apo.ansto.gov.au/dspace/handle/10238/2310 | en_AU |
dc.identifier.volume | 266 | en_AU |
dc.language.iso | en | en_AU |
dc.publisher | Elsevier | en_AU |
dc.subject | Plants | en_AU |
dc.subject | Biological localization | en_AU |
dc.subject | Biological accumulation | en_AU |
dc.subject | PIXE analysis | en_AU |
dc.subject | Bioremediation | en_AU |
dc.subject | Lyophilization | en_AU |
dc.title | Evaluation of specimen preparation techniques for micro-PIXE localisation of elements in hyperaccumulating plants | en_AU |
dc.type | Conference Paper | en_AU |
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