Nickel, Zn and Cd localisation in seeds of metal hyperaccumulators using mu-PIXE spectroscopy.

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dc.contributor.authorKachenko, AGen_AU
dc.contributor.authorBhatia, NPen_AU
dc.contributor.authorSiegele, Ren_AU
dc.contributor.authorWalsh, KBen_AU
dc.contributor.authorSingh, Ben_AU
dc.date.accessioned2010-04-06T02:21:43Zen_AU
dc.date.accessioned2010-04-30T05:05:45Zen_AU
dc.date.available2010-04-06T02:21:43Zen_AU
dc.date.available2010-04-30T05:05:45Zen_AU
dc.date.issued2009-06-15en_AU
dc.date.statistics2009-06-15en_AU
dc.description.abstractMetal hyperaccumulators are a rare group of plant species that accumulate exceptionally high concentrations of metals in above ground tissues without showing symptoms of phytotoxicity. Quantitative localisation of the accumulated metals in seed tissues is of considerable interest to help understand the eco-physiology of these unique plant species. We investigated the spatial localisation of metals within seeds of Ni hyperaccumulating Hybanthus floribundus subsp. adpressus, H. floribundus subsp. floribundus and Pimelea leptospermoides and dual-metal (Cd and Zn) hyperaccumulating Thlaspi caerulescens using quantitative micro-proton induced X-ray emission (μ-PIXE) spectroscopy. Intact seeds were hand-sectioned, sandwiched between Formvar films and irradiated using the 3 MeV high energy heavy ion microprobe at ANSTO. Elemental maps of whole H. floribundus subsp. adpressus seeds showed an average Ni concentration of 5.1 × 103 mg kg−1 dry weight (DW) with highest Ni concentration in cotyledonary tissues (7.6 × 103 mg kg−1 DW), followed by the embryonic axis (4.4 × 103 mg kg−1 DW). Nickel concentration in whole H. floribundus subsp. floribundus seeds was 3.5 × 102 mg kg−1 DW without a clear pattern of Ni localisation. The average Ni concentration in whole P. leptospermoides seeds was 2.6 × 102 mg kg−1 DW, and Ni was preferentially localised in the embryonic axis (4.3 × 102 mg kg−1 DW). In T. caerulescens, Cd concentrations were similar in cotyledon (4.5 × 103 mg kg−1 DW) and embryonic axis (3.3 × 103 mg kg−1 DW) tissues, whereas Zn was highest in cotyledonary tissues (1.5 × 103 mg kg−1 DW). In all species, the presence of the accumulated metal within the cotyledonary and embryonic axis tissues indicates that the accumulated metal was able to move apoplastically within the seed. © 2009, Elsevier Ltd.en_AU
dc.identifier.citationKachenko, A. G., Bhatia, N. P., Siegele, R., Walsh, K. B., & Singh, B. (2009). Nickel, Zn and Cd localisation in seeds of metal hyperaccumulators using mu-PIXE spectroscopy. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 267(12-13), 2176-2180. doi:10.1016/j.nimb.2009.03.059en_AU
dc.identifier.govdoc1370en_AU
dc.identifier.issn0168-583Xen_AU
dc.identifier.issue12-13en_AU
dc.identifier.journaltitleNuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atomsen_AU
dc.identifier.pagination2176-2180en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.nimb.2009.03.059en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/3042en_AU
dc.identifier.volume267en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectPIXE analysisen_AU
dc.subjectHeavy metalsen_AU
dc.subjectNickelen_AU
dc.subjectZincen_AU
dc.subjectCadmiumen_AU
dc.subjectSeedsen_AU
dc.titleNickel, Zn and Cd localisation in seeds of metal hyperaccumulators using mu-PIXE spectroscopy.en_AU
dc.typeJournal Articleen_AU
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