Determination of Na+ binding parameters by relaxation analysis of selected 23Na NMR coherences: RNA, BSA and SDS

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dc.contributor.authorTorres, AMen_AU
dc.contributor.authorPhilp, DJen_AU
dc.contributor.authorKemp-Harper, Ren_AU
dc.contributor.authorGarvey, CJen_AU
dc.contributor.authorKuchel, PWen_AU
dc.date.accessioned2009-12-08en_AU
dc.date.accessioned2010-04-30T05:05:12Zen_AU
dc.date.available2009-12-08en_AU
dc.date.available2010-04-30T05:05:12Zen_AU
dc.date.issued2005-03en_AU
dc.date.statistics2005-03en_AU
dc.description.abstractNuclear magnetic resonance provides several unique means of investigating the interactions between different inorganic ions and various macromolecules. 23Na is a quadrupolar nucleus, meaning that relaxation analysis of the various coherences allows the measurement of its binding to biological macromolecules. In this study, we analyzed the quadrupolar relaxation of 23Na+ longitudinal magnetization and single- and triple-quantum coherences in aqueous systems containing RNA, bovine serum albumin and sodium dodecyl sulfate micelles. The effectiveness of the James-Noggle method for determining binding constants was evaluated in these systems, and also the applicability of various 23Na coherences in providing information on the extent and affinity of binding to the three different classes of biomolecules. © 2004, John Wiley & Sons Ltd.en_AU
dc.identifier.citationTorres, A. M., Philp, D. J., Kemp-Harper, R., Garvey, C., & Kuchel, P. W. (2005). Determination of Na+ binding parameters by relaxation analysis of selected Na-23 NMR coherences: RNA, BSA and SDS. Magnetic Resonance in Chemistry, 43(3), 217-224. doi:10.1002/mrc.1541en_AU
dc.identifier.govdoc1340en_AU
dc.identifier.issn0749-1581en_AU
dc.identifier.issue3en_AU
dc.identifier.journaltitleMagnetic Resonance in Chemistryen_AU
dc.identifier.pagination217-224en_AU
dc.identifier.urihttp://dx.doi.org/10.1002/mrc.1541en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2630en_AU
dc.identifier.volume43en_AU
dc.language.isoenen_AU
dc.publisherJohn Wiley & Sonsen_AU
dc.subjectNuclear magnetic resonanceen_AU
dc.subjectRNAen_AU
dc.subjectSodiumen_AU
dc.subjectBinding energyen_AU
dc.subjectRelaxationen_AU
dc.subjectBiosynthesisen_AU
dc.titleDetermination of Na+ binding parameters by relaxation analysis of selected 23Na NMR coherences: RNA, BSA and SDSen_AU
dc.typeJournal Articleen_AU
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