Human erythrocyte flickering: temperature, ATP concentration, water transport, and cell aging, plus a computer simulation

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dc.contributor.authorSzekely, Den_AU
dc.contributor.authorYau, TWen_AU
dc.contributor.authorKuchel, PWen_AU
dc.date.accessioned2009-10-22T05:45:31Zen_AU
dc.date.accessioned2010-04-30T05:04:57Zen_AU
dc.date.available2009-10-22T05:45:31Zen_AU
dc.date.available2010-04-30T05:04:57Zen_AU
dc.date.issued2009-09en_AU
dc.date.statistics2009-09en_AU
dc.description.abstractImages of human erythrocytes from a healthy donor were recorded under differential interference contrast (DIC) microscopy; they were acquired rapidly (similar to 336 Hz) and the intensity of the centermost pixel of each cell was recorded for similar to 60 s (20,000 values). Various techniques were used to analyze the data, including detrended fluctuation analysis (DFA) and multiscale entropy (MSE); however, power spectrum analysis was deemed the most appropriate for metrifying and comparing results. This analysis was used to compare cells from young and old populations, and after perturbing normal conditions, with changes in temperature, adenosine triphosphate (ATP) concentration (using NaF, an inhibitor of glycolysis, and alpha-toxin, a pore-forming molecule used to permeabilize red cells to ATP), and water transport rates [using glycerol, and p-chloromercuriphenylsulfonic acid (pCMBS) to inhibit aquaporins, AQPs]. There were measurable differences in the membrane fluctuation characteristics in populations of young and old cells, but there was no significant change in the flickering time series on changing the temperature of an individual cell, by depleting it of ATP, or by competing with the minor water exchange pathway via AQP3 using glycerol. However, pCMBS, which inhibits AQP1, the major water exchange pathway, inhibited flickering in all cells, and yet it was restored by the membrane intercalating species dibutyl phthalate (DBP). We developed a computer model to simulate acquired displacement spectral time courses and to evaluate various methods of data analysis, and showed how the flexibility of the membrane, as defined in the model, affects the flickering time course. © 2009, Springer.en_AU
dc.identifier.citationSzekely, D., Yau, T. W., & Kuchel, P. W. (2009). Human erythrocyte flickering: temperature, ATP concentration, water transport, and cell aging, plus a computer simulation. European Biophysics Journal with Biophysics Letters, 38(7), 923-939. doi:10.1007/s00249-009-0473-6en_AU
dc.identifier.govdoc1324en_AU
dc.identifier.issn0175-7571en_AU
dc.identifier.issue7en_AU
dc.identifier.journaltitleEuropean Biophysics Journal with Biophysics Lettersen_AU
dc.identifier.pagination923-939en_AU
dc.identifier.urihttp://dx.doi.org/10.1007/s00249-009-0473-6en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2065en_AU
dc.identifier.volume38en_AU
dc.language.isoenen_AU
dc.publisherSpringeren_AU
dc.subjectErythrocytesen_AU
dc.subjectATPen_AU
dc.subjectComputerized simulationen_AU
dc.subjectTemperature rangeen_AU
dc.subjectAgingen_AU
dc.subjectBlood cellsen_AU
dc.titleHuman erythrocyte flickering: temperature, ATP concentration, water transport, and cell aging, plus a computer simulationen_AU
dc.typeJournal Articleen_AU
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