Changes in molecular composition and packing during lipid membrane reconstitution from phospholipid-surfactant micelles

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dc.contributor.authorLee, CSen_AU
dc.contributor.authorWacklin, HPen_AU
dc.contributor.authorBain, CDen_AU
dc.date.accessioned2009-10-22T05:16:38Zen_AU
dc.date.accessioned2010-04-30T05:04:56Zen_AU
dc.date.available2009-10-22T05:16:38Zen_AU
dc.date.available2010-04-30T05:04:56Zen_AU
dc.date.issued2009-02-07en_AU
dc.date.statistics2009-02-07en_AU
dc.description.abstractTotal internal reflection (TIR) Raman spectroscopy was used to investigate changes in the properties of phospholipid bilayers at the quartz-water interface during the process of membrane reconstitution from lipid-detergent micelles. The non-ionic detergent beta-D-dodecyl maltoside (beta-DDM) was used in 9 : 1 ratio to deuterated and non-deuterated dipalmitoylphosphatidyl choline (DPPC and d(75)-DPPC) to form detergent-lipid micelles. Exposure of the quartz surface to decreasing concentrations of such detergent-lipid solutions resulted in the formation of a d(75)-DPPC bilayer indistinguishable from that of a bilayer formed by fusion of small unilamellar vesicles of the pure lipid, indicating that the use of detergent micelles does not alter the packing or conformation of lipids, or leave a residue of surfactant in the bilayer. Polarised measurements of d(75)DPPC spectra in the C-H and C-D regions at each step of the process showed that the lipid surface density increases as the detergent is removed by rinsing and replaced by more lipid. The absence of shifts in the frequencies demonstrates that DPPC is in a gel-like conformation even at high relative surfactant fractions. The TIR geometry also allowed us to record high resolution spectra in the low frequency C-D bending region of d(75)-DPPC, which showed that the lipid molecules have an upright orientation with respect to the support surface. © 2009, Royal Society of Chemistryen_AU
dc.identifier.citationLee, C., Wacklin, H., & Bain, C. D. (2009). Changes in molecular composition and packing during lipid membrane reconstitution from phospholipid-surfactant micelles. Soft Matter, 5(3), 568-575. doi:10.1039/b812768aen_AU
dc.identifier.govdoc1322en_AU
dc.identifier.issn1744-683Xen_AU
dc.identifier.issue3en_AU
dc.identifier.journaltitleSoft Matteren_AU
dc.identifier.pagination568-575en_AU
dc.identifier.urihttp://dx.doi.org/10.1039/b812768aen_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2063en_AU
dc.identifier.volume5en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectRaman spectroscopyen_AU
dc.subjectPhospholipidsen_AU
dc.subjectMembranesen_AU
dc.subjectDetergentsen_AU
dc.subjectQuartzen_AU
dc.subjectWateren_AU
dc.titleChanges in molecular composition and packing during lipid membrane reconstitution from phospholipid-surfactant micellesen_AU
dc.typeJournal Articleen_AU
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