A DNA-based assay for toxic chemicals in wastewater

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dc.contributor.authorForeman, ALen_AU
dc.contributor.authorPhilips, Len_AU
dc.contributor.authorKanellis, VGen_AU
dc.contributor.authorHammoudeh, Den_AU
dc.contributor.authorNaumann, Cen_AU
dc.contributor.authorWong, HKYen_AU
dc.contributor.authorChisari, Ren_AU
dc.contributor.authorHibbert, DBen_AU
dc.contributor.authorLee, GSHen_AU
dc.contributor.authorPatra, Ren_AU
dc.contributor.authorJulli, Men_AU
dc.contributor.authorChapman, Jen_AU
dc.contributor.authorCooke, ARen_AU
dc.contributor.authordos Remedios, CGen_AU
dc.date.accessioned2012-04-19T05:54:16Zen_AU
dc.date.available2012-04-19T05:54:16Zen_AU
dc.date.issued2011-08-01en_AU
dc.date.statistics2012-04-19en_AU
dc.description.abstractChemical toxicants, particularly metal ions, are a major contaminant in global waterways. Live-organism bioassays used to monitor chemical toxicants commonly involve measurements of activity or survival of a freshwater cladoceran (Ceriodaphnia dubia) or light emitted by the marine bacterium Vibrio fischeri, used in the commercial Microtox (R) bioassay. Here we describe a novel molecule-based assay system employing DNA as the chemical biosensor. Metals bind to DNA, causing structural changes that expel a bound (intercalated) fluorescent reporter dye. Analyses of test data using 48 wastewater samples potentially contaminated by metal ions show that the DNA-dye assay results correlate with those from C. dubia and Microtox bioassays. All three assays exhibit additive, antagonistic, and synergistic responses that cannot be predicted by knowing individual metal concentrations. Analyses of metals in these samples imply the presence of chemical toxicants other than metal ions. The DNA-dye assay is robust, has a 12-month shelf life, and is only slightly affected by sample pH in the range 4 to 9. The assay is completed in a matter of minutes, and its portability makes it well suited as a screening assay for use in the field. We conclude that the DNA-dye test is a surrogate bioassay suitable for screening chemical toxicity. Environ. Toxicol. Chem. 2011;30:1810-1818. (C) 2011 SETACen_AU
dc.identifier.citationForeman, A. L., Phillips, L., Kanellis, V. G., Hammoudeh, D., Naumann, C., Wong, H., Chisari, R., Hibbert, D. B., Lee, G. S. H., Patra, R., Julli, M., Chapman, J., Cooke, A. R., dos Remedios, C. G. (2011). A DNA-based assay for toxic chemicals in wastewater. Environmental Toxicology and Chemistry 30(8), 1810-1818. doi:10.1002/etc.568en_AU
dc.identifier.govdoc4078en_AU
dc.identifier.issn0730-7268en_AU
dc.identifier.issue8en_AU
dc.identifier.journaltitleEnvironmental Toxicology and Chemistryen_AU
dc.identifier.pagination1810-1818en_AU
dc.identifier.urihttp://dx.doi.org/10.1002/etc.568en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/4179en_AU
dc.identifier.volume30en_AU
dc.language.isoenen_AU
dc.publisherWiley-Blackwellen_AU
dc.subjectWaste wateren_AU
dc.subjectToxic materialsen_AU
dc.subjectDNA base transitionsen_AU
dc.subjectHeavy metalsen_AU
dc.subjectCadmiumen_AU
dc.subjectPollutantsen_AU
dc.titleA DNA-based assay for toxic chemicals in wastewateren_AU
dc.typeJournal Articleen_AU
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