Effect of acidification on elemental and isotopic compositions of sediment organic matter and macro-invertebrate muscle tissues in food web research

dc.contributor.authorMazumder, Den_AU
dc.contributor.authorIles, Jen_AU
dc.contributor.authorKelleway, JJen_AU
dc.contributor.authorKobayashi, Ten_AU
dc.contributor.authorKnowles, Len_AU
dc.contributor.authorSaintilan, Nen_AU
dc.contributor.authorHollins, SEen_AU
dc.date.accessioned2010-11-22T04:27:34Zen_AU
dc.date.available2010-11-22T04:27:34Zen_AU
dc.date.issued2010-10-30en_AU
dc.date.statistics2010-10-30en_AU
dc.description.abstractStable isotope techniques in food web studies often focus on organic carbon in food sources which are subsequently assimilated in the tissue of consumer organisms through diet. The presence of non-dietary carbonates in bulk samples can affect their δ13C values, altering how their results are interpreted. Acidification of samples is a common practice to eliminate any inorganic carbon present prior to analysis. We examined the effects of pre-analysis acidification on two size fractions of sediment organic matter (SOM) from marine and freshwater wetlands and pure muscle tissue of a common freshwater invertebrate (Cherax destructor). The elemental content and isotopic ratios of carbon and nitrogen were compared between paired samples of acidified and control treatments. Our results showed that acidification does not affect the elemental or isotopic values of freshwater SOM. In the marine environment acidification depleted the δ13C and δ15N values of the fine fraction of saltmarsh and δ15N values of mangrove fine SOM. Whilst acidification did not change the elemental content of invertebrate muscle tissue, the δ13C and δ15N values were affected. We recommend to researchers considering using acidification techniques on material prepared for stable isotope analysis that a formal assessment of the effect of acidification on their particular sample type should be undertaken. Further detailed investigation to understand the impact of acidification on elemental and isotopic values of organic matter and muscular tissues is required. © 2010, Wiley-Blackwell. The definitive version is available at www3.interscience.wiley.comen_AU
dc.identifier.citationMazumder, D., Iles, J., Kelleway, J., Kobayashi, T., Knowles, L., Saintilan, N., & Hollins, S. E. (2010). Effect of acidification on elemental and isotopic compositions of sediment organic matter and macro-invertebrate muscle tissues in food web research. Rapid Communications in Mass Spectrometry, 24(20), 2938-2942. doi:10.1002/rcm.4729en_AU
dc.identifier.govdoc3065en_AU
dc.identifier.issn0951-4198en_AU
dc.identifier.issue20en_AU
dc.identifier.journaltitleRapid Communications in Mass Spectrometryen_AU
dc.identifier.pagination2938-2942en_AU
dc.identifier.urihttp://dx.doi.org/10.1002/rcm.4729en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2867en_AU
dc.identifier.volume24en_AU
dc.language.isoenen_AU
dc.publisherWiley-Blackwellen_AU
dc.subjectStable isotopesen_AU
dc.subjectAcidificationen_AU
dc.subjectOrganic matteren_AU
dc.subjectMusclesen_AU
dc.subjectFooden_AU
dc.subjectIsotope ratioen_AU
dc.titleEffect of acidification on elemental and isotopic compositions of sediment organic matter and macro-invertebrate muscle tissues in food web researchen_AU
dc.typeJournal Articleen_AU
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