Probing solid-liquid interfaces using radiotracer technology: characterization of functionalized microspheres.

Simple item page
dc.contributor.authorHuang, Sen_AU
dc.contributor.authorJoso, Ren_AU
dc.contributor.authorFuchs, Aen_AU
dc.contributor.authorBarner, Len_AU
dc.contributor.authorSmith, SVen_AU
dc.date.accessioned2010-10-20T00:52:21Zen_AU
dc.date.available2010-10-20T00:52:21Zen_AU
dc.date.issued2008-08-26en_AU
dc.date.statistics2008-08-26en_AU
dc.description.abstractThis study explores the use of nuclear probes (radiolabeling techniques) for the quantification of available, reactive groups on solid surfaces and their role in the engineering of materials for biosensor applications. Microspheres were synthesized with reactive carboxylate groups as conjugation sites for proteins. The carboxylate groups were reacted with three bifunctional ligands, sarar, aminobenzyl-cyclen, and aminobenzyl-dota. The reaction conditions used were similar to that employed for the conjugation of proteins and optimized for the concentration of reactants (bifunctional ligand and EDC), pH, and time at 21°C. Of the three bifunctional ligands conjugated to the microsphere, sarar proved to be the most efficient. Optimum reaction conditions employed molar ratios of 1:20:100 for microspheres (estimated carboxylate groups of 1.1 × 10−7 per milligram of microspheres):sarar:EDC at pH 5.0 for 1 h, followed by 57/natCo radiolabeling at pH 7.0. The study demonstrates how nuclear probes with high specificity and sensitiviy can provide invaluable information on available reactive sites on solid surfaces at extremely low concentrations (>10−10 M) in a range of media. More importantly the nuclear probes enable the characterization of the surfaces of new materials in a non-destructive manner under conditions relevant to the engineering of the materials and ultimately the desired biosensor. © 2008, American Chemical Societyen_AU
dc.identifier.citationHuang, S., Joso, R., Fuchs, A., Barner, L., & Smith, S. V. (2008). Probing solid-liquid interfaces using radiotracer technology: characterization of functionalized microspheres. Chemistry of Materials, 20(16), 5375-5380. doi:10.1021/cm800248sen_AU
dc.identifier.govdoc2863en_AU
dc.identifier.issn0897-4756en_AU
dc.identifier.issue16en_AU
dc.identifier.journaltitleChemistry of Materialsen_AU
dc.identifier.pagination5375-5380en_AU
dc.identifier.urihttp://dx.doi.org/10.1021/cm800248sen_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2704en_AU
dc.identifier.volume20en_AU
dc.language.isoenen_AU
dc.publisherAmerican Chemical Societyen_AU
dc.subjectTracer techniquesen_AU
dc.subjectRadiopharmaceuticalsen_AU
dc.subjectSurfacesen_AU
dc.subjectMicrospheresen_AU
dc.subjectLigandsen_AU
dc.subjectProbesen_AU
dc.titleProbing solid-liquid interfaces using radiotracer technology: characterization of functionalized microspheres.en_AU
dc.typeJournal Articleen_AU
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal Articles