Evaluation of radioisotope quality aspects for preparation of high specific activity [Ga-68]-NOTA-AnnexinA1.

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dc.contributor.authorFuchs, Aen_AU
dc.contributor.authorGreguric, Ien_AU
dc.contributor.authorRoe, Gen_AU
dc.date.accessioned2010-10-17T23:18:11Zen_AU
dc.date.available2010-10-17T23:18:11Zen_AU
dc.date.issued2010-09-08en_AU
dc.date.statistics2010-09-08en_AU
dc.description.abstractThe bi-functional chelator NOTA-(p-Bn)-NCS permits radio-labelling heat sensitive proteins with Ga-68 (t1/2=68 min) for positron emission tomography. Complexation at room temperature (RT) completes within minutes and in vivo transmetalation is negligible. Here, influences of trace metal cations, on radiochemical yield (RCY) and specific radioactivity (SRA) are assessed. Conjugation of NOTA-(p-Bn)-NCS to AnnexinA1 was performed at varying stoichiometries and conjugates purified by size exclusion high-performance liquid chromatography. Available complexation sites per protein were identified by colorimetric assay and titration with carrier added Ga-67. The complexation of Ga-68 by NOTA-AnnexinA1 at RT and 37°C was systematically studied with and without addition of competing trace metal cations. Ga-67 was used for confirmation of observed trends. Integrity of the radio-conjugate was assessed by addition of up to 104 fold excess of metal cations or apo-transferin and exposure to human serum at 37°C. Gallium-68 gave RCY of N99% within minutes at RT whereas, Ga-67 yielded max 85% dropping as the stock decayed. Presence of Fe(III) showed significant influence on RCY. Once formed, the radio-conjugate showed negligible loss of radioactivity under even the most extreme conditions investigated. SRA and RCY of the radio-conjugate depend significantly on absence of particularly Fe(III) during complexation. © 2020 Elsevier B.Ven_AU
dc.identifier.citationFuchs, A., Greguric, I., & Roe, G. (2010). Evaluation of radioisotope quality aspects for preparation of high specific activity [Ga-68]-NOTA-AnnexinA1. Poster presented to the International Symposium on Technetium and Other Radiometals in Chemistry and Medicine (TERACHEM 2010), 8th - 11th September 2010. Forum Brixen: Bressanone (Bolzano), Italy. In Nuclear Medicine and Biology, 37(6), 692. doi:10.1016/j.nucmedbio.2010.04.148en_AU
dc.identifier.conferenceenddate11 September 2010en_AU
dc.identifier.conferencenameInternational Symposium on Technetium and Other Radiometals in Chemistry and Medicine (TERACHEM 2010)en_AU
dc.identifier.conferenceplaceBressanone (Bolzano), Italyen_AU
dc.identifier.conferencestartdate8 September 2010en_AU
dc.identifier.govdoc2822en_AU
dc.identifier.issn0969-8051en_AU
dc.identifier.issue6en_AU
dc.identifier.journaltitleNuclear Medicine and Biologyen_AU
dc.identifier.pagination692en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.nucmedbio.2010.04.148en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2681en_AU
dc.identifier.volume37en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectPositron computed tomographyen_AU
dc.subjectRadioactivityen_AU
dc.subjectHigh-performance liquid chromatographyen_AU
dc.subjectProteinsen_AU
dc.subjectGallium 68en_AU
dc.subjectComplexometryen_AU
dc.titleEvaluation of radioisotope quality aspects for preparation of high specific activity [Ga-68]-NOTA-AnnexinA1.en_AU
dc.typeConference Posteren_AU
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