Self-assembly of protein monolayers engineered for improved monoclonal immunoglobulin G binding

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dc.contributor.authorLe Brun, APen_AU
dc.contributor.authorShah, DSHen_AU
dc.contributor.authorAthey, Den_AU
dc.contributor.authorHolt, SAen_AU
dc.contributor.authorLakey, JHen_AU
dc.date.accessioned2011-12-14T00:42:06Zen_AU
dc.date.available2011-12-14T00:42:06Zen_AU
dc.date.issued2011-08-01en_AU
dc.date.statistics2011-12-14en_AU
dc.description.abstractBacterial outer membrane proteins, along with a filling lipid molecule can be modified to form stable self-assembled monolayers on gold. The transmembrane domain of Escherichia coli outer membrane protein A has been engineered to create a scaffold protein to which functional motifs can be fused. In earlier work we described the assembly and structure of an antibody-binding array where the Z domain of Staphylococcus aureus protein A was fused to the scaffold protein. Whilst the binding of rabbit polyclonal immunoglobulin G (IgG) to the array is very strong, mouse monoclonal IgG dissociates from the array easily. This is a problem since many immunodiagnostic tests rely upon the use of mouse monoclonal antibodies. Here we describe a strategy to develop an antibody-binding array that will bind mouse monoclonal IgG with lowered dissociation from the array. A novel protein consisting of the scaffold protein fused to two pairs of Z domains separated by a long flexible linker was manufactured. Using surface plasmon resonance the self-assembly of the new protein on gold and the improved binding of mouse monoclonal IgG were demonstrated. © The Authors - This is an open access article distributed under the Creative Commons Attribution Licenseen_AU
dc.identifier.citationLe Brun, A.P., Shah, D.S.H., Athey, D., Holt, S.A., Lakey, J.H. (2011). Self-assembly of protein monolayers engineered for improved monoclonal immunoglobulin G binding. International Journal of Molecular Sciences, 12(8), 5157-5167. doi:10.3390/ijms12085157en_AU
dc.identifier.govdoc3871en_AU
dc.identifier.issn1661-6596en_AU
dc.identifier.issue8en_AU
dc.identifier.journaltitleInternational Journal of Molecular Sciencesen_AU
dc.identifier.pagination5157-5167en_AU
dc.identifier.urihttp://dx.doi.org/10.3390/ijms12085157en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/3948en_AU
dc.identifier.volume12en_AU
dc.language.isoenen_AU
dc.publisherMDPI Publishingen_AU
dc.subjectMembrane proteinsen_AU
dc.subjectGolden_AU
dc.subjectImmunoglobulinsen_AU
dc.subjectResonanceen_AU
dc.subjectSurfacesen_AU
dc.subjectMoleculesen_AU
dc.titleSelf-assembly of protein monolayers engineered for improved monoclonal immunoglobulin G bindingen_AU
dc.typeJournal Articleen_AU
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