The interaction of cubosomes with supported phospholipid bilayers using neutron reflectometry and QCM-D

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dc.contributor.authorShen, HHen_AU
dc.contributor.authorHartley, PGen_AU
dc.contributor.authorJames, Men_AU
dc.contributor.authorNelson, Aen_AU
dc.contributor.authorDefendi, Hen_AU
dc.contributor.authorMcLean, KMen_AU
dc.date.accessioned2011-12-01T04:57:29Zen_AU
dc.date.available2011-12-01T04:57:29Zen_AU
dc.date.issued2011-09-21en_AU
dc.date.statistics2011-12-01en_AU
dc.description.abstractWe present the results of a study of the interaction of lyotropic liquid crystalline dispersions with supported lipid bilayers based on 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) using a quartz crystal microbalance with dissipation monitoring (QCM-D) and neutron reflectometry (NR). We investigated two types of phytantriol-based cubosome formulations, with subtly different internal nanostructures, with one formulation incorporating 2.5% of the biological lipid di-palmitoylphosphatidylserine (DPPS). The QCM-D data showed that cubosomes do not directly attach to the silica supporting surface but they can accumulate on the model membrane, confirming that there is an attractive interaction between POPC bilayers and the cubosome formulations. We have further used NR to quantify the amount of cubosomes adsorbed on the supported POPC bilayers and to examine the structural rearrangement of cubosomes on interaction with the supported lipid bilayer. The data show that the DPPS-containing cubosomes accumulate at the bilayer surface continuously for 15 hours. Pure phytantriol cubosomes accumulated over a longer time period (36 hours), but accumulated to a lesser degree overall. Furthermore, NR data revealed lipid exchange and structural rearrangements for both types of cubosomes, however, for the DPPS-containing cubosomes, these processes were greater in magnitude and faster. Confocal microscopy analysis of cubosome interactions with HeLa cells in vitro, showed increased membrane affinity for the DPPS-containing formulations, which were consistent with the NR and QCM-D observations. We interpret these observations as suggesting that membrane accumulation, cellular uptake and cytotoxicity of cubosome formulations are directly related to their DPPS content, and that this may be the result of increased propensity for liquid crystalline structural rearrangement, as postulated previously (H. H. Shen et al., Biomaterials, 2010, 31, 9473).© 2011, Royal Society of Chemistryen_AU
dc.identifier.citationShen, H.H., Hartley, P.G., James, M., Nelson, A., Defendi, H., McLean, K.M. (2011). The interaction of cubosomes with supported phospholipid bilayers using neutron reflectometry and QCM-D. Soft Matter, 7(18), 8041-8049. doi:10.1039/c1sm05287jen_AU
dc.identifier.govdoc3839en_AU
dc.identifier.issn1744-683Xen_AU
dc.identifier.issue18en_AU
dc.identifier.journaltitleSoft Matteren_AU
dc.identifier.pagination8041-8049en_AU
dc.identifier.urihttp://dx.doi.org/10.1039/c1sm05287jen_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/3900en_AU
dc.identifier.volume7en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectNeutron reflectorsen_AU
dc.subjectPhospholipidsen_AU
dc.subjectMicroscopyen_AU
dc.subjectReflectivityen_AU
dc.subjectNanostructuresen_AU
dc.subjectSurfacesen_AU
dc.titleThe interaction of cubosomes with supported phospholipid bilayers using neutron reflectometry and QCM-Den_AU
dc.typeJournal Articleen_AU
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