Chemical alterations to murine brain tissue induced by formation fixation: implications for biospectroscopic imaging and mapping studies of disease pathogenesis

Simple item page
dc.contributor.authorHackett, MJen_AU
dc.contributor.authorMcQuillan, JAen_AU
dc.contributor.authorEl-Assaad, Fen_AU
dc.contributor.authorAitken, JBen_AU
dc.contributor.authorLevina, Aen_AU
dc.contributor.authorCohen, DDen_AU
dc.contributor.authorSiegele, Ren_AU
dc.contributor.authorCarter, EAen_AU
dc.contributor.authorGrau, GEen_AU
dc.contributor.authorHunt, NHen_AU
dc.contributor.authorLay, PAen_AU
dc.date.accessioned2011-10-11T04:53:57Zen_AU
dc.date.available2011-10-11T04:53:57Zen_AU
dc.date.issued2011-07-21en_AU
dc.date.statistics2011-10-11en_AU
dc.description.abstractUnderstanding biochemical mechanisms and changes associated with disease conditions and, therefore, development of improved clinical treatments, is relying increasingly on various biochemical mapping and imaging techniques on tissue sections. However, it is essential to be able to ascertain whether the sampling used provides the full biochemical information relevant to the disease and is free from artefacts. A multi-modal micro-spectroscopic approach, including FTIR imaging and PIXE elemental mapping, has been used to study the molecular and elemental profile within cryofixed and formalin-fixed murine brain tissue sections. The results provide strong evidence that amino acids, carbohydrates, lipids, phosphates, proteins and ions, such as Cl(-) and K(+), leach from tissue sections into the aqueous fixative medium during formalin fixation of the sections. Large changes in the concentrations and distributions of most of these components are also observed by washing in PBS even for short periods. The most likely source of the chemical species lost during fixation is the extra-cellular and intra-cellular fluid of tissues. The results highlight that, at best, analysis of formalin-fixed tissues gives only part of the complete biochemical "picture'' of a tissue sample. Further, this investigation has highlighted that significant lipid peroxidation/oxidation may occur during formalin fixation and that the use of standard histological fixation reagents can result in significant and differential metal contamination of different regions of tissue sections. While a consistent and reproducible fixation method may be suitable for diagnostic purposes, the findings of this study strongly question the use of formalin fixation prior to spectroscopic studies of the molecular and elemental composition of biological samples, if the primary purpose is mechanistic studies of disease pathogenesis.© 2011, Royal Society of Chemistryen_AU
dc.identifier.citationHackett, M. J., McQuillan, J. A., El-Assaad, F., Aitken, J. B., Levina, A., Cohen, D. D., Siegele, R., Carter, E. A., Grau, G. E., Hunt, N. H., & Lay, P. A. (2011). Chemical alterations to murine brain tissue induced by formation fixation: implications for biospectroscopic imaging and mapping studies of disease pathogenesis. Analyst, 136(14); 2941-2952. doi:10.1039/c0an00269ken_AU
dc.identifier.govdoc3543en_AU
dc.identifier.issn0003-2654en_AU
dc.identifier.issue14en_AU
dc.identifier.journaltitleAnalysten_AU
dc.identifier.pagination2941-2952en_AU
dc.identifier.urihttp://dx.doi.org/10.1039/c0an00269ken_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/3816en_AU
dc.identifier.volume136en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectBrainen_AU
dc.subjectAnimal tissuesen_AU
dc.subjectMappingen_AU
dc.subjectBiochemistryen_AU
dc.subjectDiseasesen_AU
dc.subjectPathogenesisen_AU
dc.titleChemical alterations to murine brain tissue induced by formation fixation: implications for biospectroscopic imaging and mapping studies of disease pathogenesisen_AU
dc.typeJournal Articleen_AU
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal Articles