Biogenic Pt uptake and nanoparticle formation in Medicago sativa and Brassica juncea

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dc.contributor.authorBali, Ren_AU
dc.contributor.authorSiegele, Ren_AU
dc.contributor.authorHarris, ATen_AU
dc.date.accessioned2011-01-18T04:06:43Zen_AU
dc.date.available2011-01-18T04:06:43Zen_AU
dc.date.issued2010-10en_AU
dc.date.statistics2010-10en_AU
dc.description.abstractThe ability of the facultative metallophyte plants, Medicago sativa (M. sativa) and Brassica juncea (B. juncea) to accumulate and translocate platinum (Pt) from aqueous substrates is reported. The influence of Pt concentration in the substrate (5, 10, 20, 40 and 80 ppm), exposure time (24, 48 and 72 h) and substrate pH (2, 3, 5, 7 and 9) was determined. In both plants the concentration of Pt increased with substrate concentration and exposure time. Greater accumulation was detected in the roots of M. sativa than B. juncea, up to a maximum of 94.19 mg Pt g−1 (dry biomass) compared with 38.5 mg Pt g−1 (dry biomass) following exposure to 80 ppm Pt after 72 h exposure, respectively. However, at lower substrate concentrations (5 and 20 ppm) greater quantities of Pt were detected in the shoots of B. juncea, ranging between 0.02 and 0.32 mg Pt g−1 (dry biomass) at 5 ppm across the different time intervals studied, compared with 0.02−0.14 mg Pt g−1 (dry biomass) for M. sativa, suggesting B. juncea to be a better translocator of Pt under idealised conditions at low concentrations. Higher Pt uptake was also observed in acidic media, with a maximum at pH 2 for M. sativa and pH 3 for B. juncea, indicating the role of net surface charge on the bioaccumulation of Pt. Once sequestered Pt(II) was reduced to Pt(0) due to the action of local metabolites. TEM images of M. sativa root samples showed the in vivo formation of Pt nanoparticles between 3 and 100 nm in size and of varying morphologies in the epidermal root cells. In vivo Pt distribution profiles were assessed using proton induced X-ray emission (μ-PIXE) spectroscopy, which showed even distribution across all tissue systems (epidermal, cortical and vascular) within the roots of both M. sativa and B. juncea. © 2010, Springer. The original publication is available at www.springerlink.comen_AU
dc.identifier.citationBali, R., Siegele, R., & Harris, A. T. (2010). Biogenic Pt uptake and nanoparticle formation in Medicago sativa and Brassica juncea. Journal of Nanoparticle Research, 12(8), 3087-3095. doi:10.1007/s11051-010-9904-7en_AU
dc.identifier.govdoc3137en_AU
dc.identifier.issn1388-0764en_AU
dc.identifier.issue8en_AU
dc.identifier.journaltitleJournal of Nanoparticle Researchen_AU
dc.identifier.pagination3087-3095en_AU
dc.identifier.urihttp://dx.doi.org/10.1007/s11051-010-9904-7en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2938en_AU
dc.identifier.volume12en_AU
dc.language.isoenen_AU
dc.publisherSpringeren_AU
dc.subjectPlatinumen_AU
dc.subjectTranslocationen_AU
dc.subjectPlantsen_AU
dc.subjectBiological accumulationen_AU
dc.subjectPIXE analysisen_AU
dc.subjectRootsen_AU
dc.titleBiogenic Pt uptake and nanoparticle formation in Medicago sativa and Brassica junceaen_AU
dc.typeJournal Articleen_AU
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