Specific cellular water dynamics observed in vivo by neutron scattering and NMR

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dc.contributor.authorJasnin, Men_AU
dc.contributor.authorStadler, Aen_AU
dc.contributor.authorTehei, Men_AU
dc.contributor.authorZaccai, Gen_AU
dc.date.accessioned2010-09-20T05:43:16Zen_AU
dc.date.available2010-09-20T05:43:16Zen_AU
dc.date.issued2010-09-21en_AU
dc.date.statistics2010-09-21en_AU
dc.description.abstractNeutron scattering, by using deuterium labelling, revealed how intracellular water dynamics, measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely permits the determination of motions on the molecular length (~ ångstrøm) and time (pico- to nanosecond) scales. In the bacterial and human cells, intracellular water beyond the hydration shells of cytoplasmic macromolecules and membrane faces flows as freely as liquid water. It is not "tamed" by confinement. In contrast, in the extreme halophile archaeon, in addition to free and hydration water an intracellular water component was observed with significantly slowed down translational diffusion. The results are discussed and compared to observations in E. coli and Haloarcula marismortui by deuteron spin relaxation in NMR—a method that is sensitive to water rotational dynamics on a wide range of time scales. © 2010, Royal Society of Chemistryen_AU
dc.identifier.citationJasnin, M., Stadler, A., Tehei, M., & Zaccai, G. (2010). Specific cellular water dynamics observed in vivo by neutron scattering and NMR. Physical Chemistry Chemical Physics, 12(35), 10154-10160. doi:10.1039/c0cp01048ken_AU
dc.identifier.govdoc2684en_AU
dc.identifier.issn1463-9076en_AU
dc.identifier.issue35en_AU
dc.identifier.journaltitlePhysical Chemistry Chemical Physicsen_AU
dc.identifier.pagination10154-10160en_AU
dc.identifier.urihttp://dx.doi.org/10.1039/c0cp01048ken_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2524en_AU
dc.identifier.volume12en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectScatteringen_AU
dc.subjectWateren_AU
dc.subjectBlood cellsen_AU
dc.subjectDeuteriumen_AU
dc.subjectNuclear magnetic resonanceen_AU
dc.subjectEscherichia colien_AU
dc.titleSpecific cellular water dynamics observed in vivo by neutron scattering and NMRen_AU
dc.typeJournal Articleen_AU
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