A neutron reflectivity study of the interfacial and thermal behaviour of surface-attached hairpin DNA

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dc.contributor.authorKjallman, THMen_AU
dc.contributor.authorNelson, Aen_AU
dc.contributor.authorJames, Men_AU
dc.contributor.authorDura, JAen_AU
dc.contributor.authorTravas-Sejdic, Jen_AU
dc.contributor.authorMcGillivray, DJen_AU
dc.date.accessioned2011-11-29T05:05:26Zen_AU
dc.date.available2011-11-29T05:05:26Zen_AU
dc.date.issued2011-01-01en_AU
dc.date.statistics2011-11-29en_AU
dc.description.abstractMixed self-assembled monolayers (mSAMs) have been successfully utilised as platforms for gene sensors, employing optical as well as electrochemical means of detection. Probe density is one of the most important parameters in the construction of such a sensor and thus a fundamental understanding of the structure within the mSAM is vital. In this work, the interfacial behaviour of mixed SAMs, where short PEG oligomers co-adsorbed to the surface with hairpin structured oligonucleotide (ODN) probes, has been investigated. The neutron reflectivity of the mixed SAMs was measured at differing HPP : PEG ratios, and through two routes of formation, to elucidate the effect of controlled HPP surface density on surface conformation of the probes and on the final hybridised ODN-HPP construct. General conclusions regarding the structure of the investigated SAMs could be drawn from determined thickness and volume fraction values and conformational changes in the mSAM, induced by hybridisation with complementary ODN, were also detected. An investigation of the melting behaviour of the surface-attached HPPs was also conducted with polarised neutron reflectivity and clear signs of melting were observed in the reflectivity and the SLD profiles around 45 [degree]C.© 2011, Royal Society of Chemistryen_AU
dc.identifier.citationKjallman, T.H.M., Nelson, A., James, M., Dura, J.A., Travas-Sejdic, J., & McGillivray, D.J. (2011). A neutron reflectivity study of the interfacial and thermal behaviour of surface-attached hairpin DNA, Soft Matter, 7(10); 5020-5029. doi:10.1039/c0sm01284jen_AU
dc.identifier.govdoc3874en_AU
dc.identifier.issn1744-683Xen_AU
dc.identifier.issue10en_AU
dc.identifier.journaltitleSoft Matteren_AU
dc.identifier.pagination5020-5029en_AU
dc.identifier.urihttp://dx.doi.org/10.1039/c0sm01284jen_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/3893en_AU
dc.identifier.volume7en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectSensorsen_AU
dc.subjectSpectroscopyen_AU
dc.subjectDNAen_AU
dc.subjectProbesen_AU
dc.subjectThermal analysisen_AU
dc.subjectDNA hybridizationen_AU
dc.titleA neutron reflectivity study of the interfacial and thermal behaviour of surface-attached hairpin DNAen_AU
dc.typeJournal Articleen_AU
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