Composition and Asymmetry in Supported Membranes Formed by Vesicle Fusion

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dc.contributor.authorWacklin, HPen_AU
dc.date.accessioned2011-12-01T03:57:46Zen_AU
dc.date.available2011-12-01T03:57:46Zen_AU
dc.date.issued2011-06-21en_AU
dc.date.statistics2011-12-01en_AU
dc.description.abstractThe structure and formation of supported membranes at silica surfaces by vesicle fusion was investigated by neutron reflectivity and quartz crystal microbalance (QCM-D) measurements. The structure of equimolar phospholipid mixtures of DLPC?DPPC, DMPC?DPPC, and DOPC?DPPC depends intricately on the vesicle deposition conditions. The supported bilayer membranes exhibit varying degrees of compositional asymmetry between the monolayer leaflets, which can be modified by the deposition temperature as well as the salt concentration of the vesicle solution. The total lipid composition of the supported bilayers differs from the composition of the vesicles in solution, and the monolayer proximal to the silica surface is always enriched in DPPC compared to the distal monolayer. The results, which show unambiguougsly that some exchange and rearrangement of lipids occur during vesicle deposition, can be rationalized by considering the effects of salt screening and temperature on the rates of lipid exchange, rearrangement, and vesicle adsorption, but there is also an intricate dependence on the lipid?lipid interactions. Thus, although both symmetric and asymmetric supported bilayers can be prepared from vesicles, the optimal conditions are sensitive to the lipid composition of the system. © 2011, Royal Society of Chemistryen_AU
dc.identifier.citationWacklin, H. P. (2011). Composition and asymmetry in supported membranes formed by vesicle fusion. Langmuir, 27(12), 7698-7707. doi:10.1021/la200683een_AU
dc.identifier.govdoc3813en_AU
dc.identifier.issn0743-7463en_AU
dc.identifier.issue12en_AU
dc.identifier.journaltitleLangmuiren_AU
dc.identifier.pagination7698-7707en_AU
dc.identifier.urihttp://dx.doi.org/10.1021/la200683een_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/3897en_AU
dc.identifier.volume27en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectAsymmetryen_AU
dc.subjectMembranesen_AU
dc.subjectSpectroscopyen_AU
dc.subjectMicroscopyen_AU
dc.subjectSilicaen_AU
dc.subjectCholesterolen_AU
dc.titleComposition and Asymmetry in Supported Membranes Formed by Vesicle Fusionen_AU
dc.typeJournal Articleen_AU
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